TY - JOUR T1 - Cytoskeletal Stability in the Auditory Organ <em>In Vivo</em>: RhoA Is Dispensable for Wound Healing but Essential for Hair Cell Development JF - eneuro JO - eNeuro DO - 10.1523/ENEURO.0149-17.2017 VL - 4 IS - 5 SP - ENEURO.0149-17.2017 AU - Tommi Anttonen AU - Ilya Belevich AU - Maarja Laos AU - Anni Herranen AU - Eija Jokitalo AU - Cord Brakebusch AU - Ulla Pirvola Y1 - 2017/09/01 UR - http://www.eneuro.org/content/4/5/ENEURO.0149-17.2017.abstract N2 - Wound healing in the inner ear sensory epithelia is performed by the apical domains of supporting cells (SCs). Junctional F-actin belts of SCs are thin during development but become exceptionally thick during maturation. The functional significance of the thick belts is not fully understood. We have studied the role of F-actin belts during wound healing in the developing and adult cochlea of mice in vivo. We show that the thick belts serve as intracellular scaffolds that preserve the positions of surviving cells in the cochlear sensory epithelium. Junctions associated with the thick F-actin belts did not readily disassemble during wound healing. To compensate for this, basolateral membranes of SCs participated in the closure of surface breach. Because not only neighboring but also distant SCs contributed to wound healing by basolateral protrusions, this event appears to be triggered by contact-independent diffusible signals. In the search for regulators of wound healing, we inactivated RhoA in SCs, which, however, did not limit wound healing. RhoA inactivation in developing outer hair cells (OHCs) caused myosin II delocalization from the perijunctional domain and apical cell-surface enlargement. These abnormalities led to the extrusion of OHCs from the epithelium. These results demonstrate the importance of stability of the apical domain, both in wound repair by SCs and in development of OHCs, and that only this latter function is regulated by RhoA. Because the correct cytoarchitecture of the cochlear sensory epithelium is required for normal hearing, the stability of cell apices should be maintained in regenerative and protective interventions. ER -