RT Journal Article SR Electronic T1 NEUROD2 Regulates Stim1 Expression and Store-Operated Calcium Entry in Cortical Neurons JF eneuro JO eNeuro FD Society for Neuroscience SP ENEURO.0255-16.2017 DO 10.1523/ENEURO.0255-16.2017 VO 4 IS 1 A1 Guner, Gokhan A1 Guzelsoy, Gizem A1 Isleyen, Fatma Sadife A1 Sahin, Gulcan Semra A1 Akkaya, Cansu A1 Bayam, Efil A1 Kotan, Eser Ilgin A1 Kabakcioglu, Alkan A1 Ince-Dunn, Gulayse YR 2017 UL http://www.eneuro.org/content/4/1/ENEURO.0255-16.2017.abstract AB Calcium signaling controls many key processes in neurons, including gene expression, axon guidance, and synaptic plasticity. In contrast to calcium influx through voltage- or neurotransmitter-gated channels, regulatory pathways that control store-operated calcium entry (SOCE) in neurons are poorly understood. Here, we report a transcriptional control of Stim1 (stromal interaction molecule 1) gene, which is a major sensor of endoplasmic reticulum (ER) calcium levels and a regulator of SOCE. By using a genome-wide chromatin immunoprecipitation and sequencing approach in mice, we find that NEUROD2, a neurogenic transcription factor, binds to an intronic element within the Stim1 gene. We show that NEUROD2 limits Stim1 expression in cortical neurons and consequently fine-tunes the SOCE response upon depletion of ER calcium. Our findings reveal a novel mechanism that regulates neuronal calcium homeostasis during cortical development.