TY - JOUR T1 - Modulation of Rhythmic Activity in Mammalian Spinal Networks Is Dependent on Excitability State JF - eneuro JO - eNeuro DO - 10.1523/ENEURO.0368-16.2017 VL - 4 IS - 1 SP - ENEURO.0368-16.2017 AU - Simon A. Sharples AU - Patrick J. Whelan Y1 - 2017/01/01 UR - http://www.eneuro.org/content/4/1/ENEURO.0368-16.2017.abstract N2 - Neuromodulators play an important role in activating rhythmically active motor networks; however, what remains unclear are the network interactions whereby neuromodulators recruit spinal motor networks to produce rhythmic activity. Evidence from invertebrate systems has demonstrated that the effect of neuromodulators depends on the pre-existing state of the network. We explored how network excitation state affects the ability of dopamine to evoke rhythmic locomotor activity in the neonatal mouse isolated spinal cord. We found that dopamine can evoke unique patterns of motor activity that are dependent on the excitability state of motor networks. Different patterns of motor activity ranging from tonic, nonrhythmic activity to multirhythmic, nonlocomotor activity to locomotor activity were produced by altering global motor network excitability through manipulations of the extracellular potassium and bath NMDA concentration. A similar effect was observed when network excitation was manipulated during an unstable multirhythm evoked by a low concentration (15 µm) of 5-HT, suggesting that our results are not neuromodulator specific. Our data show in vertebrate systems that modulation is a two-way street and that modulatory actions are largely influenced by the network state. The level of network excitation can account for variability between preparations and is an additional factor to be considered when circuit elements are removed from the network. ER -