TY - JOUR T1 - Regulation of Physical Microglia–Neuron Interactions by Fractalkine Signaling after Status Epilepticus JF - eneuro JO - eNeuro DO - 10.1523/ENEURO.0209-16.2016 VL - 3 IS - 6 SP - ENEURO.0209-16.2016 AU - Ukpong B. Eyo AU - Jiyun Peng AU - Madhuvika Murugan AU - Mingshu Mo AU - Almin Lalani AU - Ping Xie AU - Pingyi Xu AU - David J. Margolis AU - Long-Jun Wu Y1 - 2016/11/01 UR - http://www.eneuro.org/content/3/6/ENEURO.0209-16.2016.abstract N2 - Microglia, the resident immune cells of the brain, perform elaborate surveillance in which they physically interact with neuronal elements. A novel form of microglia–neuron interaction named microglial process convergence (MPC) toward neuronal axons and dendrites has recently been described. However, the molecular regulators and pathological relevance of MPC have not been explored. Here, using high-resolution two-photon imaging in vivo and ex vivo, we observed a dramatic increase in MPCs after kainic acid– or pilocarpine-induced experimental seizures that was reconstituted after glutamate treatment in slices from mice. Interestingly, a deficiency of the fractalkine receptor (CX3CR1) decreased MPCs, whereas fractalkine (CX3CL1) treatment increased MPCs, suggesting that fractalkine signaling is a critical regulator of these microglia–neuron interactions. Furthermore, we found that interleukin-1β was necessary and sufficient to trigger CX3CR1-dependent MPCs. Finally, we show that a deficiency in fractalkine signaling corresponds with increased seizure phenotypes. Together, our results identify the neuroglial CX3CL1–CX3CR1 communication axis as a modulator of potentially neuroprotective microglia–neuron physical interactions during conditions of neuronal hyperactivity. ER -