RT Journal Article
SR Electronic
T1 SLC26A11 (KBAT) in Purkinje Cells Is Critical for Inhibitory Transmission and Contributes to Locomotor Coordination
JF eneuro
JO eneuro
FD Society for Neuroscience
SP ENEURO.0028-16.2016
DO 10.1523/ENEURO.0028-16.2016
VO 3
IS 3
A1 Rahmati, Negah
A1 Vinueza Veloz, Maria Fernanda
A1 Xu, Jie
A1 Barone, Sharon
A1 Rodolfo Ben Hamida, Nahuel
A1 Schonewille, Martijn
A1 Hoebeek, Freek E.
A1 Soleimani, Manoocher
A1 De Zeeuw, Chris I.
YR 2016
UL http://www.eneuro.org/content/3/3/ENEURO.0028-16.2016.abstract
AB Chloride homeostasis determines the impact of inhibitory synaptic transmission and thereby mediates the excitability of neurons. Even though cerebellar Purkinje cells (PCs) receive a pronounced inhibitory GABAergic input from stellate and basket cells, the role of chloride homeostasis in these neurons is largely unknown. Here we studied at both the cellular and systems physiological level the function of a recently discovered chloride channel, SLC26A11 or kidney brain anion transporter (KBAT), which is prominently expressed in PCs. Using perforated patch clamp recordings of PCs, we found that a lack of KBAT channel in PC-specific KBAT KO mice (L7-KBAT KOs) induces a negative shift in the reversal potential of chloride as reflected in the GABAA-receptor-evoked currents, indicating a decrease in intracellular chloride concentration. Surprisingly, both in vitro and in vivo PCs in L7-KBAT KOs showed a significantly increased action potential firing frequency of simple spikes, which correlated with impaired motor performance on the Erasmus Ladder. Our findings support an important role for SLC26A11 in moderating chloride homeostasis and neuronal activity in the cerebellum.