RT Journal Article SR Electronic T1 Identification of Immediate Early Genes in the Nervous System of Snail Helix lucorum JF eneuro JO eNeuro FD Society for Neuroscience SP ENEURO.0416-18.2019 DO 10.1523/ENEURO.0416-18.2019 VO 6 IS 3 A1 Xu, Chuan A1 Li, Qian A1 Efimova, Olga A1 Jiang, Xi A1 Petrova, Marina A1 K. Vinarskaya, Alia A1 Kolosov, Peter A1 Aseyev, Nikolay A1 Koshkareva, Kira A1 Ierusalimsky, Victor N. A1 Balaban, Pavel M. A1 Khaitovich, Philipp YR 2019 UL http://www.eneuro.org/content/6/3/ENEURO.0416-18.2019.abstract AB Immediate early genes (IEGs) are useful markers of neuronal activation and essential components of neuronal response. While studies of gastropods have provided many insights into the basic learning and memory mechanisms, the genome-wide assessment of IEGs has been mainly restricted to vertebrates. In this study, we identified IEGs in the terrestrial snail Helix lucorum. In the absence of the genome, we conducted de novo transcriptome assembly using reads with short and intermediate lengths cumulatively covering more than 98 billion nucleotides. Based on this assembly, we identified 37 proteins corresponding to contigs differentially expressed (DE) in either the parietal ganglia (PaG) or two giant interneurons located within the PaG of the snail in response to the neuronal stimulation. These proteins included homologues of well-known mammalian IEGs, such as c-jun/jund, C/EBP, c-fos/fosl2, and Egr1, as well as homologues of genes not yet implicated in the neuronal response.