RT Journal Article
SR Electronic
T1 Gordon Holmes Syndrome Model Mice Exhibit Alterations in Microglia, Age, and Sex-Specific Disruptions in Cognitive and Proprioceptive Function
JF eneuro
JO eNeuro
FD Society for Neuroscience
SP ENEURO.0074-23.2023
DO 10.1523/ENEURO.0074-23.2023
VO 11
IS 1
A1 George, Arlene J.
A1 Wei, Wei
A1 Pyaram, Dhanya N.
A1 Gomez, Morgan
A1 Shree, Nitheyaa
A1 Kadirvelu, Jayashree
A1 Lail, Hannah
A1 Wanders, Desiree
A1 Murphy, Anne Z.
A1 Mabb, Angela M.
YR 2024
UL http://www.eneuro.org/content/11/1/ENEURO.0074-23.2023.abstract
AB Gordon Holmes syndrome (GHS) is a neurological disorder associated with neuroendocrine, cognitive, and motor impairments with corresponding neurodegeneration. Mutations in the E3 ubiquitin ligase RNF216 are strongly linked to GHS. Previous studies show that deletion of Rnf216 in mice led to sex-specific neuroendocrine dysfunction due to disruptions in the hypothalamic–pituitary–gonadal axis. To address RNF216 action in cognitive and motor functions, we tested Rnf216 knock-out (KO) mice in a battery of motor and learning tasks for a duration of 1 year. Although male and female KO mice did not demonstrate prominent motor phenotypes, KO females displayed abnormal limb clasping. KO mice also showed age-dependent strategy and associative learning impairments with sex-dependent alterations of microglia in the hippocampus and cortex. Additionally, KO males but not females had more negative resting membrane potentials in the CA1 hippocampus without any changes in miniature excitatory postsynaptic current (mEPSC) frequencies or amplitudes. Our findings show that constitutive deletion of Rnf216 alters microglia and neuronal excitability, which may provide insights into the etiology of sex-specific impairments in GHS.