<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Paliarin, Franciely</style></author><author><style face="normal" font="default" size="100%">Duplantis, Chelsea</style></author><author><style face="normal" font="default" size="100%">Jones, Andrea F.</style></author><author><style face="normal" font="default" size="100%">Cucinello-Ragland, Jessica</style></author><author><style face="normal" font="default" size="100%">Basavanhalli, Samhita</style></author><author><style face="normal" font="default" size="100%">Blaze, Emily</style></author><author><style face="normal" font="default" size="100%">Doré, Evan</style></author><author><style face="normal" font="default" size="100%">Neel, Anna Isabella</style></author><author><style face="normal" font="default" size="100%">Sun, Haiguo</style></author><author><style face="normal" font="default" size="100%">Chen, Rong</style></author><author><style face="normal" font="default" size="100%">Edwards, Scott</style></author><author><style face="normal" font="default" size="100%">Gilpin, Nicholas W.</style></author><author><style face="normal" font="default" size="100%">Messing, Robert O.</style></author><author><style face="normal" font="default" size="100%">Maiya, Rajani</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">A Cre Driver Line for Genetic Targeting of Kappa Opioid Receptor Expressing Cells</style></title><secondary-title><style face="normal" font="default" size="100%">eneuro</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2023-07-01 00:00:00</style></date></pub-dates></dates><elocation-id><style  face="normal" font="default" size="100%">ENEURO.0043-23.2023</style></elocation-id><doi><style  face="normal" font="default" size="100%">10.1523/ENEURO.0043-23.2023</style></doi><volume><style face="normal" font="default" size="100%">10</style></volume><issue><style face="normal" font="default" size="100%">7</style></issue><abstract><style  face="normal" font="default" size="100%">Here we describe the generation and characterization of a Cre knock-in mouse line that harbors a Cre insertion in the 3′UTR of the κ opioid receptor gene (Oprk1) locus and provides genetic access to populations of κ opioid receptor (KOR)-expressing neurons throughout the brain. Using a combination of techniques including RNA in situ hybridization and immunohistochemistry, we report that Cre is expressed with high fidelity in KOR-expressing cells throughout the brain in this mouse line. We also provide evidence that Cre insertion does not alter basal KOR function. Baseline anxiety-like behaviors and nociceptive thresholds are unaltered in Oprk1-Cre mice. Chemogenetic activation of KOR-expressing cells in the basolateral amygdala (BLAKOR cells) resulted in several sex-specific effects on anxiety-like and aversive behaviors. Activation led to decreased anxiety-like behavior on the elevated plus maze and increased sociability in female but not in male Oprk1-Cre mice. Activation of BLAKOR cells also attenuated KOR agonist-induced conditioned place aversion (CPA) in male Oprk1-Cre mice. Overall, these results suggest a potential role for BLAKOR cells in regulating anxiety-like behaviors and KOR-agonist mediated CPA. In summary, these results provide evidence for the utility of the newly generated Oprk1-Cre mice in assessing localization, anatomy, and function of KOR circuits throughout the brain.</style></abstract></record></records></xml>