TY - JOUR T1 - Chronic Intermittent Ethanol Exposure Dysregulates Nucleus Basalis Magnocellularis Afferents in the Basolateral Amygdala JF - eneuro JO - eNeuro DO - 10.1523/ENEURO.0164-22.2022 VL - 9 IS - 6 SP - ENEURO.0164-22.2022 AU - Sarah E. Sizer AU - Michaela E. Price AU - Brian C. Parrish AU - Samuel H. Barth AU - Chelcie F. Heaney AU - Kimberly F. Raab-Graham AU - Brian A. McCool Y1 - 2022/11/01 UR - http://www.eneuro.org/content/9/6/ENEURO.0164-22.2022.abstract N2 - Nucleus basalis magnocellularis (NBM) cholinergic projections to the basolateral amygdala (BLA) regulate the acquisition and consolidation of fear-like and anxiety-like behaviors. However, it is unclear whether the alterations in the NBM-BLA circuit promote negative affect during ethanol withdrawal (WD). Therefore, we performed ex vivo whole-cell patch-clamp electrophysiology in both the NBM and the BLA of male Sprague Dawley rats following 10 d of chronic intermittent ethanol (CIE) exposure and 24 h of WD. We found that CIE exposure and withdrawal enhanced the neuronal excitability of NBM putative “cholinergic” neurons. We subsequently used optogenetics to directly manipulate NBM terminal activity within the BLA and measure cholinergic modulation of glutamatergic afferents and BLA pyramidal neurons. Our findings indicate that CIE and withdrawal upregulate NBM cholinergic facilitation of glutamate release via activation of presynaptic nicotinic acetylcholine receptors (AChRs). Ethanol withdrawal-induced increases in NBM terminal activity also enhance BLA pyramidal neuron firing. Collectively, our results provide a novel characterization of the NBM-BLA circuit and suggest that CIE-dependent modifications to NBM afferents enhance BLA pyramidal neuron activity during ethanol withdrawal. ER -