TY - JOUR T1 - Frmpd1 Facilitates Trafficking of G-Protein Transducin and Modulates Synaptic Function in Rod Photoreceptors of Mammalian Retina JF - eneuro JO - eNeuro DO - 10.1523/ENEURO.0348-22.2022 VL - 9 IS - 5 SP - ENEURO.0348-22.2022 AU - Christie K. Campla AU - Ulisse Bocchero AU - Ryan Strickland AU - Jacob Nellissery AU - Jayshree Advani AU - Irina Ignatova AU - Dhiraj Srivastava AU - Angel M. Aponte AU - Yuchen Wang AU - Jessica Gumerson AU - Kirill Martemyanov AU - Nikolai O. Artemyev AU - Johan Pahlberg AU - Anand Swaroop Y1 - 2022/09/01 UR - http://www.eneuro.org/content/9/5/ENEURO.0348-22.2022.abstract N2 - Trafficking of transducin (Gαt) in rod photoreceptors is critical for adaptive and modulatory responses of the retina to varying light intensities. In addition to fine-tuning phototransduction gain in rod outer segments (OSs), light-induced translocation of Gαt to the rod synapse enhances rod to rod bipolar synaptic transmission. Here, we show that the rod-specific loss of Frmpd1 (FERM and PDZ domain containing 1), in the retina of both female and male mice, results in delayed return of Gαt from the synapse back to outer segments in the dark, compromising the capacity of rods to recover from light adaptation. Frmpd1 directly interacts with Gpsm2 (G-protein signaling modulator 2), and the two proteins are required for appropriate sensitization of rod-rod bipolar signaling under saturating light conditions. These studies provide insight into how the trafficking and function of Gαt is modulated to optimize the photoresponse and synaptic transmission of rod photoreceptors in a light-dependent manner. ER -