TY - JOUR T1 - Perinatal Morphine Exposure Leads to Sex-Dependent Executive Function Deficits and Microglial Changes in Mice JF - eneuro JO - eNeuro DO - 10.1523/ENEURO.0238-22.2022 VL - 9 IS - 5 SP - ENEURO.0238-22.2022 AU - Brittany L. Smith AU - Tess A. Guzman AU - Alexander H. Brendle AU - Collin J. Laaker AU - Alexis Ford AU - Adam R. Hiltz AU - Junfang Zhao AU - Kenneth D. R. Setchell AU - Teresa M. Reyes Y1 - 2022/09/01 UR - http://www.eneuro.org/content/9/5/ENEURO.0238-22.2022.abstract N2 - Children exposed prenatally to opioids are at an increased risk for behavioral problems and executive function deficits. The prefrontal cortex (PFC) and amygdala (AMG) regulate executive function and social behavior and are sensitive to opioids prenatally. Opioids can bind to toll-like receptor 4 (TLR4) to activate microglia, which may be developmentally important for synaptic pruning. Therefore, we tested the effects of perinatal morphine exposure on executive function and social behavior in male and female mouse offspring, along with microglial-related and synaptic-related outcomes. Dams were injected once daily subcutaneously with saline (n = 8) or morphine (MO; 10 mg/kg; n = 12) throughout pregestation, gestation, and lactation until offspring were weaned on postnatal day 21 (P21). Male MO offspring had impairments in attention and accuracy in the five-choice serial reaction time task, while female MO offspring were less affected. Targeted gene expression analysis at P21 in the PFC identified alterations in microglial-related and TLR4-related genes, while immunohistochemical analysis in adult brains indicated decreased microglial Iba1 and phagocytic CD68 proteins in the PFC and AMG in males, but females had an increase. Further, both male and female MO offspring had increased social preference. Overall, these data demonstrate male vulnerability to executive function deficits in response to perinatal opioid exposure and evidence for disruptions in neuron–microglial signaling. ER -