RT Journal Article SR Electronic T1 Low-Cost Platform for Multianimal Chronic Local Field Potential Video Monitoring with Graphical User Interface (GUI) for Seizure Detection and Behavioral Scoring JF eneuro JO eNeuro FD Society for Neuroscience SP ENEURO.0283-22.2022 DO 10.1523/ENEURO.0283-22.2022 VO 9 IS 5 A1 Gergely Tarcsay A1 Brittney Lee Boublil A1 Laura A. Ewell YR 2022 UL http://www.eneuro.org/content/9/5/ENEURO.0283-22.2022.abstract AB Experiments employing chronic monitoring of neurophysiological signals and video are commonly used in studies of epilepsy to characterize behavioral correlates of seizures. Our objective was to design a low-cost platform that enables chronic monitoring of several animals simultaneously, synchronizes bilateral local field potential (LFP) and video streams in real time, and parses recorded data into manageable file sizes. We present a hardware solution leveraging Intan and Open Ephys acquisition systems and a software solution implemented in Bonsai. The platform was tested in 48-h continuous recordings simultaneously from multiple mice (male and female) with chronic epilepsy. To enable seizure detection and scoring, we developed a graphical user interface (GUI) that reads the data produced by our workflow and allows a user with no coding expertise to analyze events. Our Bonsai workflow was designed to maximize flexibility for a wide variety of experimental applications, and our use of the Open Ephys acquisition board would allow for scaling recordings up to 128 channels per animal.