RT Journal Article
SR Electronic
T1 Endogenous Circadian Clock Machinery in Cortical NG2-Glia Regulates Cellular Proliferation
JF eneuro
JO eNeuro
FD Society for Neuroscience
SP ENEURO.0110-22.2022
DO 10.1523/ENEURO.0110-22.2022
VO 9
IS 5
A1 Terry Dean
A1 Aissia Victoria Koffi
A1 Evan Goldstein
A1 Javid Ghaemmaghami
A1 Vittorio Gallo
YR 2022
UL http://www.eneuro.org/content/9/5/ENEURO.0110-22.2022.abstract
AB The molecular circadian clock can be found throughout the body and is essential for the synchronizing cellular physiology with the 24 h day. However, the role of the clock in regulating the regenerative potential of the brain has not been explored. We report here that murine NG2-glia, the largest population of proliferative cells in the mature central nervous system, rhythmically express circadian clock genes in a 24 h period, including the critical clock component Bmal1 RNA and BMAL1 protein. Interestingly, daily NG2-glia proliferation preferentially occurs during the time of day in which Bmal1 expression is high, while conditional knockout of Bmal1 decreases both cortical NG2-glia density and cellular proliferation. Furthermore, in a neurotrauma model, we show that pathology-induced NG2-glia proliferation is also dependent on Bmal1 expression. Because circadian rhythm disturbances are common in neurologic disorders across the life span, including in traumatic brain injury, these findings bear significant implications for cellular regeneration in brain injuries and disease.