RT Journal Article SR Electronic T1 Sources of Variation in the Spectral Slope of the Sleep EEG JF eneuro JO eNeuro FD Society for Neuroscience SP ENEURO.0094-22.2022 DO 10.1523/ENEURO.0094-22.2022 VO 9 IS 5 A1 Kozhemiako, Nataliia A1 Mylonas, Dimitris A1 Pan, Jen Q. A1 Prerau, Michael J. A1 Redline, Susan A1 Purcell, Shaun M. YR 2022 UL http://www.eneuro.org/content/9/5/ENEURO.0094-22.2022.abstract AB The 1/f spectral slope of the electroencephalogram (EEG) estimated in the γ frequency range has been proposed as an arousal marker that differentiates wake, nonrapid eye movement (NREM) sleep, and rapid eye movement (REM) sleep. Here, we sought to replicate and extend these findings in a large sample, providing a comprehensive characterization of how slope changes with age, sex, and its test-retest reliability as well as potential confounds that could affect the slope estimation. We used 10,255 whole-night polysomnograms (PSGs) from the National Sleep Research Resource (NSRR). All preprocessing steps were performed using an open-source Luna package and the spectral slope was estimated by fitting log-log linear regression models on the absolute power from 30 to 45 Hz separately for wake, NREM, and REM stages. We confirmed that the mean spectral slope grows steeper going from wake to NREM to REM sleep. We found that the choice of mastoid referencing scheme modulated the extent to which electromyogenic, or electrocardiographic artifacts were likely to bias 30- to 45-Hz slope estimates, as well as other sources of technical, device-specific bias. Nonetheless, within individuals, slope estimates were relatively stable over time. Both cross-sectionally and longitudinal, slopes tended to become shallower with increasing age, particularly for REM sleep; males tended to show flatter slopes than females across all states. Our findings support that spectral slope can be a valuable arousal marker for both clinical and research endeavors but also underscore the importance of considering interindividual variation and multiple methodological aspects related to its estimation.