PT - JOURNAL ARTICLE AU - Luis Martinez-Ramirez AU - Andrea Slate AU - George D. Price AU - Ann-Christine Duhaime AU - Kevin J. Staley AU - Beth A. Costine-Bartell TI - Robust, Long-Term Video EEG Monitoring in a Porcine Model of Post-Traumatic Epilepsy AID - 10.1523/ENEURO.0025-22.2022 DP - 2022 Jul 01 TA - eneuro PG - ENEURO.0025-22.2022 VI - 9 IP - 4 4099 - http://www.eneuro.org/content/9/4/ENEURO.0025-22.2022.short 4100 - http://www.eneuro.org/content/9/4/ENEURO.0025-22.2022.full SO - eNeuro2022 Jul 01; 9 AB - To date, post-traumatic epilepsy (PTE) research in large-animal models has been limited. Recent advances in neocortical microscopy have made possible new insights into neocortical PTE. However, it is very difficult to engender convincing neocortical PTE in rodents. Thus, large-animal models that develop neocortical PTE may provide useful insights that also can be more comparable to human patients. Because gyrencephalic species have prolonged latent periods, long-term video EEG recording is required. Here, we report a fully subcutaneous EEG implant with synchronized video in freely ambulatory swine for up to 13 months during epileptogenesis following bilateral cortical impact injuries or sham surgery The advantages of this system include the availability of a commercially available system that is simple to install, a low failure rate after surgery for EEG implantation, radiotelemetry that enables continuous monitoring of freely ambulating animals, excellent synchronization to video to EEG, and a robust signal-to-noise ratio. The disadvantages of this system in this species and age are the accretion of skull bone, which entirely embedded a subset of skull screws and EEG electrodes, and the inability to rearrange the EEG electrode array. These disadvantages may be overcome by splicing a subdural electrode strip to the electrode leads so that skull growth is less likely to interfere with long-term signal capture and by placing two implants for a more extensive montage. This commercially available system in this bilateral cortical impact swine model may be useful to a wide range of investigators studying epileptogenesis in PTE.