RT Journal Article SR Electronic T1 Identifying Inputs to Visual Projection Neurons in Drosophila Lobula by Analyzing Connectomic Data JF eneuro JO eNeuro FD Society for Neuroscience SP ENEURO.0053-22.2022 DO 10.1523/ENEURO.0053-22.2022 VO 9 IS 2 A1 Ryosuke Tanaka (田中涼介) A1 Damon A. Clark YR 2022 UL http://www.eneuro.org/content/9/2/ENEURO.0053-22.2022.abstract AB Electron microscopy (EM)-based connectomes provide important insights into how visual circuitry of fruit fly Drosophila computes various visual features, guiding and complementing behavioral and physiological studies. However, connectomic analyses of the lobula, a neuropil putatively dedicated to detecting object-like features, remains underdeveloped, largely because of incomplete data on the inputs to the brain region. Here, we attempted to map the columnar inputs into the Drosophila lobula neuropil by performing connectivity-based and morphology-based clustering on a densely reconstructed connectome dataset. While the dataset mostly lacked visual neuropils other than lobula, which would normally help identify inputs to lobula, our clustering analysis successfully extracted clusters of cells with homogeneous connectivity and morphology, likely representing genuine cell types. We were able to draw a correspondence between the resulting clusters and previously identified cell types, revealing previously undocumented connectivity between lobula input and output neurons. While future, more complete connectomic reconstructions are necessary to verify the results presented here, they can serve as a useful basis for formulating hypotheses on mechanisms of visual feature detection in lobula.