TY - JOUR T1 - Opioid and Sucrose Craving Are Accompanied by Unique Behavioral and Affective Profiles after Extended Abstinence in Male and Female Rats JF - eneuro JO - eNeuro DO - 10.1523/ENEURO.0515-21.2022 VL - 9 IS - 2 SP - ENEURO.0515-21.2022 AU - Hannah L. Mayberry AU - Heather A. DeSalvo AU - Charlotte C. Bavley AU - Sara H. Downey AU - Cindy Lam AU - Charita Kunta AU - Ricardo P. Fortuna AU - Priya H. Doshi AU - Elizabeth B. Smedley AU - Mathieu E. Wimmer Y1 - 2022/03/01 UR - http://www.eneuro.org/content/9/2/ENEURO.0515-21.2022.abstract N2 - Incubation of craving refers to the intensification of drug-seeking behavior in response to reward-paired cues over the course of abstinence. In rodents, craving and drug-seeking behaviors have been measured by an increase in lever pressing in the absence of reinforcer availability in response to cue presentations. However, craving in rodents is difficult to define and little is known about the behavioral signatures that accompany increased drug-seeking behavior measured by lever pressing. The affective components of relapse are also important, but understudied in rodents. Hormonal fluctuations influence craving for psychostimulants, but little is known about the impact of the estrous cycle on opioid-seeking behavior. This study sought to delineate the behavioral and affective signatures associated with craving, and to examine the influence of the female estrous cycle on craving. Male and female rats underwent 10 d of intravenous opioid self-administration. Separate cohorts of control rats self-administered oral sucrose, a natural nondrug reward. Cue-induced seeking tests were conducted after 1 or 30d of forced abstinence. These sessions were recorded and scored for overall locomotion, instances of sniffing, grooming, or hyperactivity. Ultrasonic vocalizations (USVs) were also recorded to determine affective profiles that accompany opioid seeking. Although active lever presses and overall locomotion increased unanimously over extended abstinence from heroin and sucrose, a sex- and reinforcer-specific behavioral and affective signature of craving emerged. Furthermore, although the female estrous cycle did not affect taking or seeking, it appears to influence more granular behaviors. ER -