RT Journal Article SR Electronic T1 Disruption of VGLUT1 in Cholinergic Medial Habenula Projections Increases Nicotine Self-Administration JF eneuro JO eNeuro FD Society for Neuroscience SP ENEURO.0481-21.2021 DO 10.1523/ENEURO.0481-21.2021 VO 9 IS 1 A1 Souter, Elizabeth A. A1 Chen, Yen-Chu A1 Zell, Vivien A1 Lallai, Valeria A1 Steinkellner, Thomas A1 Conrad, William S. A1 Wisden, William A1 Harris, Kenneth D. A1 Fowler, Christie D. A1 Hnasko, Thomas S. YR 2022 UL http://www.eneuro.org/content/9/1/ENEURO.0481-21.2021.abstract AB Cholinergic projections from the medial habenula (MHb) to the interpeduncular nucleus (IPN) have been studied for their complex contributions to nicotine addiction and have been implicated in nicotine reinforcement, aversion, and withdrawal. While it has been established that MHb cholinergic projections corelease glutamate, no direct evidence has demonstrated a role for this glutamate projection in nicotine consumption. In the present study, a novel floxed Slc17a7 [vesicular glutamate transporter 1 (VGLUT1)] mouse was generated and used to create conditional knock-out (cKO) mice that lack VGLUT1 in MHb cholinergic neurons. Loss of Slc17a7 expression in ventral MHb cholinergic neurons was validated using fluorescent in situ hybridization, and immunohistochemistry was used to demonstrate a corresponding reduction of VGLUT1 protein in cholinergic terminals in the IPN. We also used optogenetics-assisted electrophysiology to evoke EPSCs in IPN and observed a reduction of glutamatergic currents in the cKO, supporting the functional disruption of VGLUT1 in MHb to IPN synapses. cKO mice exhibited no gross phenotypic abnormalities and displayed normal thigmotaxis and locomotor behavior in the open-field assay. When trained to lever press for food, there was no difference between control and cKO. However, when tested in a nicotine self-administration procedure, we found that the loss of VGLUT1-mediated glutamate corelease led to increased responding for nicotine. These findings indicate that glutamate corelease from ventral MHb cholinergic neurons opposes nicotine self-administration, and provide additional support for targeting this synapse to develop potential treatments for nicotine addiction.