PT  - JOURNAL ARTICLE
AU  - Ztaou, Samira
AU  - Oh, Soo Jung
AU  - Tepler, Sophia
AU  - Fleury, Sixtine
AU  - Matamales, Miriam
AU  - Bertran-Gonzalez, Jesus
AU  - Chuhma, Nao
AU  - Rayport, Stephen
TI  - Single Dose of Amphetamine Induces Delayed Subregional Attenuation of Cholinergic Interneuron Activity in the Striatum
AID  - 10.1523/ENEURO.0196-21.2021
DP  - 2021 Sep 01
TA  - eneuro
PG  - ENEURO.0196-21.2021
VI  - 8
IP  - 5
4099  - http://www.eneuro.org/content/8/5/ENEURO.0196-21.2021.short
4100  - http://www.eneuro.org/content/8/5/ENEURO.0196-21.2021.full
SO  - eNeuro2021 Sep 01; 8
AB  - Psychostimulants such as amphetamine (AMPH) target dopamine (DA) neuron synapses to engender drug-induced plasticity. While DA neurons modulate the activity of striatal (Str) cholinergic interneurons (ChIs) with regional heterogeneity, how AMPH affects ChI activity has not been elucidated. Here, we applied quantitative fluorescence imaging approaches to map the dose-dependent effects of a single dose of AMPH on ChI activity at 2.5 and 24 h after injection across the mouse Str using the activity-dependent marker phosphorylated ribosomal protein S6 (p-rpS6240/244). AMPH did not affect the distribution or morphology of ChIs in any Str subregion. While AMPH at either dose had no effect on ChI activity after 2.5 h, ChI activity was dose dependently reduced after 24 h specifically in the ventral Str/nucleus accumbens (NAc), a critical site of psychostimulant action. AMPH at either dose did not affect the spontaneous firing of ChIs. Altogether this work demonstrates that a single dose of AMPH has delayed regionally heterogeneous effects on ChI activity, which most likely involves extra-Str synaptic input.