TY - JOUR T1 - Protein Nanoparticles Modified with PDGF-B as a Novel Therapy After Acute Cerebral Infarction JF - eneuro JO - eNeuro DO - 10.1523/ENEURO.0098-21.2021 VL - 8 IS - 5 SP - ENEURO.0098-21.2021 AU - Soh Takagishi AU - Koichi Arimura AU - Masaharu Murata AU - Katsuma Iwaki AU - Tomohiro Okuda AU - Keisuke Ido AU - Ataru Nishimura AU - Sayoko Narahara AU - Takahito Kawano AU - Koji Iihara Y1 - 2021/09/01 UR - http://www.eneuro.org/content/8/5/ENEURO.0098-21.2021.abstract N2 - Treatment options for cerebral infarction beyond the time window of reperfusion therapy are limited, and novel approaches are needed. PDGF-B is considered neuroprotective; however, it is difficult to administer at effective concentrations to infarct areas. Nanoparticles (NPs) are small and stable; therefore, we modified PDGF-B to the surface of naturally occurring heat shock protein NPs (HSPNPs) to examine its therapeutic effect in cerebral infarction. PDGF-B modified HSPNPs (PDGF-B HSPNPs) were injected 1 d after transient middle cerebral artery occlusion (t-MCAO) in CB-17 model mice. We analyzed the infarct volume and motor functional recovery at 3 and 7 d. PDGF-B HSPNPs were specifically distributed in the infarct area, and compared with HSPNPs alone, they significantly reduced infarct volumes and improved neurologic function 3 and 7 d after administration. PDGF-B HSPNP administration was associated with strong phosphorylation of Akt in infarct areas and significantly increased neurotrophin (NT)-3 production as well as reduced cell apoptosis compared with HSPNPs alone. Moreover, astrogliosis in peri-infarct area was significantly upregulated with PDGF-B HSPNPs compared with HSPNPs alone. Treatment with PDGF-B HSPNPs might be a novel approach for treating cerebral infarction. ER -