TY - JOUR T1 - Interhemispheric Cortico-Cortical Pathway for Sequential Bimanual Movements in Mice JF - eneuro JO - eNeuro DO - 10.1523/ENEURO.0200-21.2021 VL - 8 IS - 4 SP - ENEURO.0200-21.2021 AU - Minju Jeong AU - Hyeonsu Lee AU - Youngsoo Kim AU - Eric Hou-Jen Wang AU - Se-Bum Paik AU - Byung Kook Lim AU - Daesoo Kim Y1 - 2021/07/01 UR - http://www.eneuro.org/content/8/4/ENEURO.0200-21.2021.abstract N2 - Animals precisely coordinate their left and right limbs for various adaptive purposes. While the left and right limbs are clearly controlled by different cortical hemispheres, the neural mechanisms that determine the action sequence between them remains elusive. Here, we have established a novel head-fixed bimanual-press (biPress) sequence task in which mice sequentially press left and right pedals with their forelimbs in a predetermined order. Using this motor task, we found that the motor cortical neurons responsible for the first press (1P) also generate independent motor signals for the second press (2P) by the opposite forelimb during the movement transitions between forelimbs. Projection-specific calcium imaging and optogenetic manipulation revealed these motor signals are transferred from one motor cortical hemisphere to the other via corticocortical projections. Together, our results suggest the motor cortices coordinate sequential bimanual movements through corticocortical pathways. ER -