RT Journal Article
SR Electronic
T1 Transection of the Superior Sagittal Sinus Enables Bilateral Access to the Rodent Midline Brain Structures
JF eneuro
JO eNeuro
FD Society for Neuroscience
SP ENEURO.0146-21.2021
DO 10.1523/ENEURO.0146-21.2021
VO 8
IS 4
A1 Marcelo Dias
A1 Inês Marques-Morgado
A1 Joana E. Coelho
A1 Pedro Ruivo
A1 Luísa V. Lopes
A1 Miguel Remondes
YR 2021
UL http://www.eneuro.org/content/8/4/ENEURO.0146-21.2021.abstract
AB Stereotaxic access to brain areas underneath the superior sagittal sinus (SSS) is notoriously challenging. As a major drainage vessel, covering the whole extension of the sagittal fissure, the SSS impedes direct bilateral access to underlying regions for recording and stimulation probes, drug-delivery cannulas, and injection devices. We now describe a new method for transection and retraction of the SSS in rats, that allows the accurate placement of microinjection devices, or chronic electrode probes, while avoiding hemorrhage and the ensuing deleterious consequences for local structures, animal health, and behavior. To demonstrate the feasibility of this approach we evaluated its consequences acutely during surgery, and thereafter during surgical survival, recovery, behavioral testing, as well as postmortem analysis of histologic impact in the related brain structures of male rats. This method provides a new approach enabling direct access for manipulation and recording of activity in brain areas previously obstructed by the SSS.