PT  - JOURNAL ARTICLE
AU  - Dhriti Nagar
AU  - Tomin K. James
AU  - Ratnakar Mishra
AU  - Shrobona Guha
AU  - Shawn M. Burgess
AU  - Aurnab Ghose
TI  - The Formin Fmn2b Is Required for the Development of an Excitatory Interneuron Module in the Zebrafish Acoustic Startle Circuit
AID  - 10.1523/ENEURO.0329-20.2021
DP  - 2021 Jul 01
TA  - eneuro
PG  - ENEURO.0329-20.2021
VI  - 8
IP  - 4
4099  - http://www.eneuro.org/content/8/4/ENEURO.0329-20.2021.short
4100  - http://www.eneuro.org/content/8/4/ENEURO.0329-20.2021.full
SO  - eNeuro2021 Jul 01; 8
AB  - The formin family member Fmn2 is a neuronally enriched cytoskeletal remodeling protein conserved across vertebrates. Recent studies have implicated Fmn2 in neurodevelopmental disorders, including sensory processing dysfunction and intellectual disability in humans. Cellular characterization of Fmn2 in primary neuronal cultures has identified its function in the regulation of cell-substrate adhesion and consequently growth cone translocation. However, the role of Fmn2 in the development of neural circuits in vivo, and its impact on associated behaviors have not been tested. Using automated analysis of behavior and systematic investigation of the associated circuitry, we uncover the role of Fmn2b in zebrafish neural circuit development. As reported in other vertebrates, the zebrafish ortholog of Fmn2 is also enriched in the developing zebrafish nervous system. We find that Fmn2b is required for the development of an excitatory interneuron pathway, the spiral fiber neuron, which is an essential circuit component in the regulation of the Mauthner cell (M-cell)-mediated acoustic startle response. Consistent with the loss of the spiral fiber neurons tracts, high-speed video recording revealed a reduction in the short latency escape events while responsiveness to the stimuli was unaffected. Taken together, this study provides evidence for a circuit-specific requirement of Fmn2b in eliciting an essential behavior in zebrafish. Our findings underscore the importance of Fmn2 in neural development across vertebrate lineages and highlight zebrafish models in understanding neurodevelopmental disorders.