RT Journal Article SR Electronic T1 Swimming Exercise Promotes Post-injury Axon Regeneration and Functional Restoration through AMPK JF eneuro JO eNeuro FD Society for Neuroscience SP ENEURO.0414-20.2021 DO 10.1523/ENEURO.0414-20.2021 VO 8 IS 3 A1 Sandeep Kumar A1 Sibaram Behera A1 Atrayee Basu A1 Shirshendu Dey A1 Anindya Ghosh-Roy YR 2021 UL http://www.eneuro.org/content/8/3/ENEURO.0414-20.2021.abstract AB Restoration of lost function following a nervous system injury is limited in adulthood as the regenerative capacity of nervous system declines with age. Pharmacological approaches have not been very successful in alleviating the consequences of nervous system injury. On the contrary, physical activity and rehabilitation interventions are often beneficial to improve the health conditions in the patients with neuronal injuries. Using touch neuron circuit of Caenorhabditis elegans, we investigated the role of physical exercise in the improvement of functional restoration after axotomy. We found that a swimming session of 90 min following the axotomy of posterior lateral microtubule (PLM) neuron can improve functional recovery in larval and adult stage animals. In older age, multiple exercise sessions were required to enhance the functional recovery. Genetic analysis of axon regeneration mutants showed that exercise-mediated enhancement of functional recovery depends on the ability of axon to regenerate. Exercise promotes early initiation of regrowth, self-fusion of proximal and distal ends, as well as postregrowth enhancement of function. We further found that the swimming exercise promotes axon regeneration through the activity of cellular energy sensor AAK-2/AMPK in both muscle and neuron. Our study established a paradigm where systemic effects of exercise on functional regeneration could be addressed at the single neuron level.