PT - JOURNAL ARTICLE AU - Yee Fun Lee AU - Lavender Lariviere AU - Alyssa N. Russ AU - Sang-Zin Choi AU - Brian J. Bacskai AU - Ksenia V. Kastanenka TI - Novel Botanical Therapeutic NB-02 Effectively Treats Alzheimer’s Neuropathophysiology in an APP/PS1 Mouse Model AID - 10.1523/ENEURO.0389-20.2021 DP - 2021 May 01 TA - eneuro PG - ENEURO.0389-20.2021 VI - 8 IP - 3 4099 - http://www.eneuro.org/content/8/3/ENEURO.0389-20.2021.short 4100 - http://www.eneuro.org/content/8/3/ENEURO.0389-20.2021.full SO - eNeuro2021 May 01; 8 AB - Alzheimer’s disease (AD) is an incurable neurodegenerative disorder and a major cause of dementia. Some of the hallmarks of AD include presence of amyloid plaques in brain parenchyma, calcium dysregulation within individual neurons, and neuroinflammation. A promising therapeutic would reverse or stymie these pathophysiologies in an animal model of AD. We tested the effect of NB-02, previously known as DA-9803, a novel multimodal therapeutic, on amyloid deposition, neuronal calcium regulation and neuroinflammation in 8- to 10-month-old APP/PS1 mice, an animal model of AD. In vivo multiphoton microscopy revealed that two-month-long administration of NB-02 halted amyloid plaque deposition and cleared amyloid in the cortex. Postmortem analysis verified NB-02-dependent decrease in plaque deposition in the cortex as well as hippocampus. Furthermore, drug treatment reversed neuronal calcium elevations, thus restoring neuronal function. Finally, NB-02 restored spine density and transformed the morphology of astrocytes as well as microglia to a more phagocytic state, affecting neuroinflammation. NB-02 was effective at reversing AD neuropathophysiology in an animal model. Therefore, in addition to serving as a promising preventative agent, NB-02 holds potential as a treatment for AD in the clinic.