RT Journal Article
SR Electronic
T1 Phenotypic Differences between the Alzheimer’s Disease-Related hAPP-J20 Model and Heterozygous <em>Zbtb20</em> Knock-Out Mice
JF eneuro
JO eNeuro
FD Society for Neuroscience
SP ENEURO.0089-21.2021
DO 10.1523/ENEURO.0089-21.2021
VO 8
IS 3
A1 Gulbranson, Daniel R.
A1 Ho, Kaitlyn
A1 Yu, Gui-Qiu
A1 Yu, Xinxing
A1 Das, Melanie
A1 Shao, Eric
A1 Kim, Daniel
A1 Zhang, Weiping J.
A1 Choudhary, Krishna
A1 Thomas, Reuben
A1 Mucke, Lennart
YR 2021
UL http://www.eneuro.org/content/8/3/ENEURO.0089-21.2021.abstract
AB Diverse gene products contribute to the pathogenesis of Alzheimer’s disease (AD). Experimental models have helped elucidate their mechanisms and impact on brain functions. Human amyloid precursor protein (hAPP) transgenic mice from line J20 (hAPP-J20 mice) are widely used to simulate key aspects of AD. However, they also carry an insertional mutation in noncoding sequence of one Zbtb20 allele, a gene involved in neural development. We demonstrate that heterozygous hAPP-J20 mice have reduced Zbtb20 expression in some AD-relevant brain regions, but not others, and that Zbtb20 levels are higher in hAPP-J20 mice than heterozygous Zbtb20 knock-out (Zbtb20+/–) mice. Whereas hAPP-J20 mice have premature mortality, severe deficits in learning and memory, other behavioral alterations, and prominent nonconvulsive epileptiform activity, Zbtb20+/– mice do not. Thus, the insertional mutation in hAPP-J20 mice does not ablate the affected Zbtb20 allele and is unlikely to account for the AD-like phenotype of this model.