TY - JOUR T1 - The Contribution of Environmental Enrichment to Phenotypic Variation in Mice and Rats JF - eneuro JO - eNeuro DO - 10.1523/ENEURO.0539-20.2021 SP - ENEURO.0539-20.2021 AU - Amanda C. Kentner AU - Amanda V. Speno AU - Joanne Doucette AU - Ryland C. Roderick Y1 - 2021/02/22 UR - http://www.eneuro.org/content/early/2021/02/19/ENEURO.0539-20.2021.abstract N2 - The reproducibility and translation of neuroscience research is assumed to be undermined by introducing environmental complexity and heterogeneity. Rearing laboratory animals with minimal (if any) environmental stimulation is thought to control for biological variability but may not adequately test the robustness of our animal models. Standard laboratory housing is associated with reduced demonstrations of species typical behaviors and changes in neurophysiology that may impact the translation of research results. Modest increases in environmental enrichment (EE), mitigate against insults used to induce animal models of disease, directly calling into question the translatability of our work. This may in part underlie the disconnect between preclinical and clinical research findings. Enhancing environmental stimulation for our model organisms promotes ethological natural behaviors but may simultaneously increase phenotypic trait variability. To test this assumption, we conducted a systematic review and evaluated coefficients of variation between EE and standard housed mice and rats. Given findings of suboptimal reporting of animal laboratory housing conditions, we also developed a methodological reporting table for enrichment use in neuroscience research. Our data show that animals housed in environmental enrichment were not more variable than those in standard housing. Therefore, environmental heterogeneity introduced into the laboratory, in the form of enrichment, does not compromise data integrity. Overall, human life is complicated and by embracing such nuanced complexity into our laboratories we may paradoxically improve upon the rigor and reproducibility of our research.Significance StatementEnvironmental complexity is thought to increase phenotypic variability, undermining research translation. We conducted a systematic review and compared coefficients of variation between environmentally enriched and standard housed laboratory animals. Despite there being no differences in variability across several phenotypic traits, there are stark contrasts in the display of ethological natural behaviors between these housing conditions. Environmental enrichment is recognized as being beneficial for animal welfare and mitigates against insults used to induce animal models of disease. In contrast, standard laboratory cages are recognized as being impoverished and ‘unnatural’. From these observations, it is apparent that our current “gold standard” caging system is not a true control condition as it does not adequately test the robustness of our animal models. ER -