RT Journal Article
SR Electronic
T1 Group II Metabotropic Glutamate Receptors Modulate Sound Evoked and Spontaneous Activity in the Mouse Inferior Colliculus
JF eneuro
JO eNeuro
FD Society for Neuroscience
SP ENEURO.0328-20.2020
DO 10.1523/ENEURO.0328-20.2020
VO 8
IS 1
A1 Kristaponyte, Inga
A1 Beebe, Nichole L.
A1 Young, Jesse W.
A1 Shanbhag, Sharad J.
A1 Schofield, Brett R.
A1 Galazyuk, Alexander V.
YR 2021
UL http://www.eneuro.org/content/8/1/ENEURO.0328-20.2020.abstract
AB Little is known about the functions of Group II metabotropic glutamate receptors (mGluRs2/3) in the inferior colliculus (IC), a midbrain structure that is a major integration region of the central auditory system. We investigated how these receptors modulate sound-evoked and spontaneous firing in the mouse IC in vivo. We first performed immunostaining and tested hearing thresholds to validate vesicular GABA transporter (VGAT)-ChR2 transgenic mice on a mixed CBA/CaJ x C57BL/6J genetic background. Transgenic animals allowed for optogenetic cell-type identification. Extracellular single neuron recordings were obtained before and after pharmacological mGluR2/3 activation. We observed increased sound-evoked firing, as assessed by the rate-level functions (RLFs), in a subset of both GABAergic and non-GABAergic IC neurons following mGluR2/3 pharmacological activation. These neurons also displayed elevated spontaneous excitability and were distributed throughout the IC area tested, suggesting a widespread mGluR2/3 distribution in the mouse IC.