%0 Journal Article %A Raoul Torero Ibad %A Bilal Mazhar %A Clémentine Vincent %A Clémence Bernard %A Julie Dégardin %A Manuel Simonutti %A Thomas Lamonerie %A Ariel A. Di Nardo %A Alain Prochiantz %A Kenneth L. Moya %T OTX2 Non-Cell Autonomous Activity Regulates Inner Retinal Function %D 2020 %R 10.1523/ENEURO.0012-19.2020 %J eneuro %P ENEURO.0012-19.2020 %V 7 %N 5 %X OTX2 is a homeoprotein transcription factor expressed in photoreceptors and bipolar cells in the retina. OTX2, like many other homeoproteins, transfers between cells and exerts non-cell autonomous effects such as promoting the survival of retinal ganglion cells that do not express the protein. Here we used a genetic approach to target extracellular OTX2 in the retina by conditional expression of a secreted single-chain anti-OTX2 antibody. Compared with control mice, the expression of this antibody by parvalbumin-expressing neurons in the retina is followed by a reduction in visual acuity in 1-month-old mice with no alteration of the retinal structure or cell type number or aspect. The a-waves and b-waves measured by electroretinogram were also indistinguishable from those of control mice, suggesting no functional deficit of photoreceptors and bipolar cells. Mice expressing the OTX2-neutralizing antibody did show a significant doubling in the flicker amplitude and a reduction in oscillatory potential, consistent with a change in inner retinal function. Our results show that interfering in vivo with OTX2 non-cell autonomous activity in the postnatal retina leads to an alteration in inner retinal cell functions and causes a deficit in visual acuity. %U https://www.eneuro.org/content/eneuro/7/5/ENEURO.0012-19.2020.full.pdf