RT Journal Article
SR Electronic
T1 Developmental Changes in Dendritic Spine Morphology in the Striatum and Their Alteration in an A53T α-Synuclein Transgenic Mouse Model of Parkinson’s Disease
JF eneuro
JO eNeuro
FD Society for Neuroscience
SP ENEURO.0072-20.2020
DO 10.1523/ENEURO.0072-20.2020
VO 7
IS 4
A1 Laxmi Kumar Parajuli
A1 Ken Wako
A1 Suiki Maruo
A1 Soichiro Kakuta
A1 Tomoyuki Taguchi
A1 Masashi Ikuno
A1 Hodaka Yamakado
A1 Ryosuke Takahashi
A1 Masato Koike
YR 2020
UL http://www.eneuro.org/content/7/4/ENEURO.0072-20.2020.abstract
AB The aging process is accompanied by various neurophysiological changes, and the severity of neurodegenerative disorders such as Parkinson’s disease (PD) increases with aging. However, the precise neuroanatomical changes that accompany the aging process in both normal and pathologic conditions remain unknown. This is in part because there is a lack of high-resolution imaging tool that has the capacity to image a desired volume of neurons in a high-throughput and automated manner. In the present study, focused ion beam/scanning electron microscopy (FIB/SEM) was used to image striatal neuropil in both wild-type (WT) mice and an A53T bacterial artificial chromosome (BAC) human α-synuclein (A53T-BAC-SNCA) transgenic (Tg) mouse model of PD, at 1, 3, 6, and 22 months of age. We demonstrated that spine density gradually decreases, and average spine head volume gradually increases with age in WT mice, suggesting a homeostatic balance between spine head volume and spine density. However, this inverse relationship between spine head volume and spine density was not observed in A53T-BAC-SNCA Tg mice. Taken together, our data suggest that PD is accompanied by an abnormality in the mechanisms that control synapse growth and maturity.