%0 Journal Article
%A Tugba Guven-Ozkan
%A Germain U. Busto
%A Jae-Yoon Jung
%A Ilaria Drago
%A Ronald L. Davis
%T miR-92a Suppresses Mushroom Body-Dependent Memory Consolidation in Drosophila
%D 2020
%R 10.1523/ENEURO.0224-20.2020
%J eneuro
%P ENEURO.0224-20.2020
%V 7
%N 4
%X MicroRNAs (miRNAs) fine tune gene expression to regulate many aspects of nervous system physiology. Here, we show that miR-92a suppresses memory consolidation that occurs in the αβ and γ mushroom body neurons (MBns) of Drosophila, making miR-92a a memory suppressor miRNA. Bioinformatics analyses suggested that mRNAs encoding kinesin heavy chain 73 (KHC73), a protein that belongs to Kinesin-3 family of anterograde motor proteins, may be a functional target of miR-92a. Behavioral studies that employed expression of khc73 with and without its 3’ untranslated region (UTR) containing miR-92a target sites, luciferase assays in HEK cells with reporters containing wild-type and mutant target sequences in the khc73 3’UTR, and immunohistochemistry experiments involving KHC73 expression with and without the wild-type khc73 3’UTR, all point to the conclusion that khc73 is a major target of miR-92a in its functional role as a miRNA memory suppressor gene.
%U https://www.eneuro.org/content/eneuro/7/4/ENEURO.0224-20.2020.full.pdf