PT - JOURNAL ARTICLE AU - Raoul Torero-Ibad AU - Bilal Mazhar AU - Clémentine Vincent AU - Clémence Bernard AU - Julie Dégardin AU - Manuel Simonutti AU - Thomas Lamonerie AU - Ariel Di Nardo AU - Alain Prochiantz AU - Kenneth L. Moya TI - OTX2 non-cell autonomous activity regulates inner retinal function AID - 10.1523/ENEURO.0012-19.2020 DP - 2020 Jul 31 TA - eneuro PG - ENEURO.0012-19.2020 4099 - http://www.eneuro.org/content/early/2020/07/31/ENEURO.0012-19.2020.short 4100 - http://www.eneuro.org/content/early/2020/07/31/ENEURO.0012-19.2020.full AB - OTX2 is a homeoprotein transcription factor expressed in photoreceptors and bipolar cells in the retina. OTX2, like many other homeoproteins, transfers between cells and exerts non-cell autonomous effects such as promoting survival of retinal ganglion cells that do not express the protein. Here we used a genetic approach to target extracellular OTX2 in the retina by conditional expression of a secreted single chain anti-OTX2 antibody. Compared to control mice, the expression of this antibody by Parvalbumin-expressing neurons in the retina is followed by a reduction in visual acuity in one-month-old mice with no alteration of the retinal structure or cell type number or aspect. A- and b-waves measured by electroretinogram were also indistinguishable from control mice, suggesting no functional deficit of photoreceptors and bipolar cells. Mice expressing the OTX2-neutralizing antibody did show a significant doubling in the flicker amplitude, consistent with a change in inner retinal function. Our results show that interfering in vivo with OTX2 non-cell autonomous activity in the postnatal retina leads to an alteration in inner retinal cell functions and causes a deficit in visual acuity.Significance statement OTX2 is a homeoprotein transcription factor expressed in retinal photoreceptors and bipolar cells. Although the Otx2 locus is silent in the inner retina, the protein is detected in cells of the ganglion cell layer consistent with the ability of this class of proteins to transfer between cells. We expressed a secreted single chain antibody (scFv) against OTX2 in the retina to neutralize extracellular OTX2. Antibody expression leads to reduced visual acuity with no change in retinal structure, or photoreceptor or bipolar physiology; however, activity in the inner retina was altered. Thus, interfering with OTX2 non-cell autonomous activity in postnatal retina alters inner retinal function and causes vision loss, highlighting the physiological value of homeoprotein direct non-cell autonomous signaling.