TY - JOUR T1 - Electric field application <em>in vivo</em> regulates neural precursor cell behaviour in the adult mammalian forebrain JF - eneuro JO - eNeuro DO - 10.1523/ENEURO.0273-20.2020 SP - ENEURO.0273-20.2020 AU - Elana Sefton AU - Stephanie N. Iwasa AU - Taylor Morrison AU - Hani E. Naguib AU - Milos R. Popovic AU - Cindi M. Morshead Y1 - 2020/07/27 UR - http://www.eneuro.org/content/early/2020/07/27/ENEURO.0273-20.2020.abstract N2 - Deep brain stimulation (DBS), which uses electrical stimulation, is a well-established neurosurgical technique used to treat neurological disorders. Despite its broad therapeutic use, the effects of electrical stimulation on brain cells is not fully understood. Here, we examine the effects of electrical stimulation on neural stem and progenitor cells (collectively neural precursor cells; NPCs) C57BLJ/6 mice found in the subventricular zone (SVZ) of the adult forebrain. Previous work has demonstrated that adult-derived NPCs are electro-sensitive and undergo rapid and directed migration in response to application of clinically relevant electric fields. We examine NPC proliferation kinetics and their differentiation profile following electric field application using in vitro and in vivo assays. In vitro direct current electrical stimulation of 250 mV/mm is sufficient to elicit a 2-fold increase in the neural stem cell pool and increases neurogenesis and oligogenesis. In vivo, asymmetric biphasic electrical stimulation similarly increases the size of the NPC pool and alters neurogenesis. These findings provide insight into the effects of electrical stimulation on NPCs and suggest its potential use as a regenerative approach to neural repair.Significance Statement Electrical stimulation promotes neural precursor cell migration. In this study we demonstrate that electrical stimulation in addition to cell migration can also expand the size of the NPC pool and enhance neurogenesis, both in vitro and in vivo. Using electrical stimulation to activate neural stem cells could be a powerful tool to promote tissue repair. ER -