@article {MatosENEURO.0035-20.2020, author = {Heidi Y. Matos and David Hernandez-Pineda and Claire M. Charpentier and Allison Rusk and Joshua G. Corbin and Kevin S. Jones}, title = {Sex Differences in Biophysical Signatures across Molecularly Defined Medial Amygdala Neuronal Subpopulations}, volume = {7}, number = {4}, elocation-id = {ENEURO.0035-20.2020}, year = {2020}, doi = {10.1523/ENEURO.0035-20.2020}, publisher = {Society for Neuroscience}, abstract = {The medial amygdala (MeA) is essential for processing innate social and non-social behaviors, such as territorial aggression and mating, which display in a sex-specific manner. While sex differences in cell numbers and neuronal morphology in the MeA are well established, if and how these differences extend to the biophysical level remain unknown. Our previous studies revealed that expression of the transcription factors, Dbx1 and Foxp2, during embryogenesis defines separate progenitor pools destined to generate different subclasses of MEA inhibitory output neurons. We have also previously shown that Dbx1-lineage and Foxp2-lineage neurons display different responses to innate olfactory cues and in a sex-specific manner. To examine whether these neurons also possess sex-specific biophysical signatures, we conducted a multidimensional analysis of the intrinsic electrophysiological profiles of these transcription factor defined neurons in the male and female MeA. We observed striking differences in the action potential (AP) spiking patterns across lineages, and across sex within each lineage, properties known to be modified by different voltage-gated ion channels. To identify the potential mechanism underlying the observed lineage-specific and sex-specific differences in spiking adaptation, we conducted a phase plot analysis to narrow down putative ion channel candidates. Of these candidates, we found a subset expressed in a lineage-biased and/or sex-biased manner. Thus, our results uncover neuronal subpopulation and sex differences in the biophysical signatures of developmentally defined MeA output neurons, providing a potential physiological substrate for how the male and female MeA may process social and non-social cues that trigger innate behavioral responses.}, URL = {https://www.eneuro.org/content/7/4/ENEURO.0035-20.2020}, eprint = {https://www.eneuro.org/content/7/4/ENEURO.0035-20.2020.full.pdf}, journal = {eNeuro} }