TY - JOUR T1 - Concentration-Dependent Recruitment of Mammalian Odorant Receptors JF - eneuro JO - eNeuro DO - 10.1523/ENEURO.0103-19.2019 VL - 7 IS - 2 SP - ENEURO.0103-19.2019 AU - Xiaoyang Serene Hu AU - Kentaro Ikegami AU - Aashutosh Vihani AU - Kevin W. Zhu AU - Marcelo Zapata AU - Claire A. de March AU - Matthew Do AU - Natasha Vaidya AU - Gary Kucera AU - Cheryl Bock AU - Yue Jiang AU - Masafumi Yohda AU - Hiroaki Matsunami Y1 - 2020/03/01 UR - http://www.eneuro.org/content/7/2/ENEURO.0103-19.2019.abstract N2 - A fundamental challenge in studying principles of organization used by the olfactory system to encode odor concentration information has been identifying comprehensive sets of activated odorant receptors (ORs) across a broad concentration range inside freely behaving animals. In mammals, this has recently become feasible with high-throughput sequencing-based methods that identify populations of activated ORs in vivo. In this study, we characterized the mouse OR repertoires activated by the two odorants, acetophenone (ACT) and 2,5-dihydro-2,4,5-trimethylthiazoline (TMT), from 0.01% to 100% (v/v) as starting concentrations using phosphorylated ribosomal protein S6 capture followed by RNA-Seq. We found Olfr923 to be one of the most sensitive ORs that is enriched by ACT. Using a mouse line that genetically labels Olfr923-positive axons, we provided evidence that ACT activates the Olfr923 glomeruli in the olfactory bulb. Through molecular dynamics stimulations, we identified amino acid residues in the Olfr923 binding cavity that facilitate ACT binding. This study sheds light on the active process by which unique OR repertoires may collectively facilitate the discrimination of odorant concentrations. ER -