TY - JOUR T1 - Does birth trigger cell death in the developing brain? JF - eneuro JO - eNeuro DO - 10.1523/ENEURO.0517-19.2020 SP - ENEURO.0517-19.2020 AU - Alexandra Castillo-Ruiz AU - Taylor A. Hite AU - Dina W. Yakout AU - T. John Rosen AU - Nancy G. Forger Y1 - 2020/02/03 UR - http://www.eneuro.org/content/early/2020/02/03/ENEURO.0517-19.2020.abstract N2 - Developmental cell death eliminates half of the neurons initially generated in the mammalian brain, and occurs perinatally in many species. It is possible that the timing of neuronal cell death is developmentally programmed, and only coincidentally associated with birth. Alternatively, birth may play a role in shaping cell death. To test these competing hypotheses, we experimentally advanced or delayed birth by one day in mice (within the normal range of gestation for the species) and examined effects on the temporal pattern and magnitude (amount) of neuronal cell death, using immunohistochemical detection of activated caspase-3 as a cell death marker. In order to detect effects of subtle changes in birth timing, we focused on brain areas that exhibit sharp postnatal peaks in cell death. We find that advancing birth advances peak cell death, supporting the hypothesis that birth triggers cell death. However, a delay of birth does not delay cell death. Thus, birth can advance cell death, but if postponed, a developmental program governs. Advancing or delaying birth also caused region-specific changes in the overall magnitude of cell death. Our findings shed light on the long-standing question of what controls the timing and magnitude of developmental neuronal cell death, and position birth as an orchestrator of brain development. Because humans across the world now routinely alter birth timing, these findings may have implications for current obstetric practices.Significance Statement The importance of neuronal cell death for brain development has been recognized for decades, but it is unknown what regulates its timing, or accounts for differences in the amount of cell death between brain regions. In many species, including mice, developmental cell death occurs perinatally. We find that advancing birth by one day in mice advances patterns of cell death, but does not advance overall forebrain growth. Because humans across the world routinely alter birth timing, usually to advance birth, our findings may have implications for current obstetric practices. Birth timing also affects the magnitude of cell death in a region-specific manner, suggesting that birth has important, previously unrecognized, effects on brain development. ER -