@article {HuENEURO.0103-19.2019, author = {Xiaoyang Serene Hu and Kentaro Ikegami and Aashutosh Vihani and Kevin W. Zhu and Marcelo Zapata and Claire de March and Matthew Do and Natasha Vaidya and Gary Kucera and Cheryl Bock and Yue Jiang and Masafumi Yohda and Hiroaki Matsunami}, title = {Concentration-dependent recruitment of mammalian odorant receptors}, elocation-id = {ENEURO.0103-19.2019}, year = {2020}, doi = {10.1523/ENEURO.0103-19.2019}, publisher = {Society for Neuroscience}, abstract = {A fundamental challenge in studying principles of organization used by the olfactory system to encode odor concentration information has been identifying comprehensive sets of activated odorant receptors (ORs) across a broad concentration range inside freely behaving animals. In mammals, this has recently become feasible with high-throughput sequencing-based methods that identify populations of activated ORs in vivo. In this study, we characterized the mouse OR repertoires activated by the two odorants, acetophenone and 2,5-dihydro-2,4,5-trimethylthiazoline, from 0.01\% to 100\% (v/v) as starting concentrations using phosphorylated ribosomal protein S6 capture followed by RNA-Seq. We found Olfr923 to be one of the most sensitive ORs that is enriched by acetophenone. Using a mouse line that genetically labels Olfr923-positive axons, we provided evidence that acetophenone activates the Olfr923 glomeruli in the olfactory bulb. Through molecular dynamics stimulations, we identified amino acid residues in the Olfr923 binding cavity that facilitate acetophenone binding. This study sheds light on the active process by which unique OR repertoires may collectively facilitate the discrimination of odorant concentrations.Significance Statement The ability of animals to discriminate odors over a range of odor concentrations while recognizing concentration-invariant odor identity presents an encoding challenge for the olfactory system. To further our understanding on how animals sense odors at different concentrations, it is important to describe how odor concentration information is represented at the receptor level. Here, we establish a sensitive in vivo approach to screen populations of odorant receptors enriched in the odor-activated sensory neurons in mice. We identified comprehensive lists of enriched odorant receptors against a 10,000-fold concentration range for two odorants. Describing the concentration-dependent activation for unique populations of odorant receptors is fundamental for future studies in determining how individual odorant receptors contribute to olfactory sensitivity and odor intensity coding.}, URL = {https://www.eneuro.org/content/early/2020/01/31/ENEURO.0103-19.2019}, eprint = {https://www.eneuro.org/content/early/2020/01/31/ENEURO.0103-19.2019.full.pdf}, journal = {eNeuro} }