PT  - JOURNAL ARTICLE
AU  - K. Ouk
AU  - J. Aungier
AU  - M. Ware
AU  - A. J. Morton
TI  - Abnormal photic entrainment to phase-delaying stimuli in the R6/2 mouse model of Huntington's disease, despite retinal responsiveness to light
AID  - 10.1523/ENEURO.0088-19.2019
DP  - 2019 Nov 19
TA  - eneuro
PG  - ENEURO.0088-19.2019
4099  - http://www.eneuro.org/content/early/2019/11/19/ENEURO.0088-19.2019.short
4100  - http://www.eneuro.org/content/early/2019/11/19/ENEURO.0088-19.2019.full
AB  - The circadian clock located in the suprachiasmatic nucleus (SCN) in mammals entrains to ambient light via the retinal photoreceptors. This allows behavioural rhythms to change in synchrony with seasonal and daily changes in light period. Circadian rhythmicity is progressively disrupted in Huntington’s disease (HD) and in HD mouse models such as the transgenic R6/2 line. Although retinal afferent inputs to the SCN are disrupted in R6/2 mice at late stages, they can respond to changes in light-dark cycles, as seen in jet lag and 23-hour-day paradigms. To investigate photic entrainment and SCN function in R6/2 mice at different stages of disease, we first assessed the effect on locomotor activity of exposure to a 15-minute light pulse given at different times of the day. We then placed the mice under 5 non-standard light conditions. These were light cycle regimes (T-cycles) of T21 (10.5h light:10.5h dark), T22 (11h light:11h dark), T26 (13h light:13h dark), constant light or constant dark. We found a progressive impairment in photic synchronisation in R6/2 mice when the stimuli required the SCN to lengthen rhythms (phase-delaying light pulse, T26 or constant light), but normal synchronisation to stimuli that required the SCN to shorten rhythms (phase-advancing light pulse and T22). Despite the behavioural abnormalities, we found that Per1 and c-fos gene expression remained photo-inducible in SCN of R6/2 mice. Both the endogenous drift of the R6/2 mouse SCN to shorter periods and its inability to adapt to phase-delaying changes will contribute to the HD circadian dysfunction.