@article {ShipmanENEURO.0125-19.2019, author = {Megan L. Shipman and Gregory C. Johnson and Mark E. Bouton and John T. Green}, title = {Chemogenetic silencing of prelimbic cortex to anterior dorsomedial striatum projection attenuates operant responding}, elocation-id = {ENEURO.0125-19.2019}, year = {2019}, doi = {10.1523/ENEURO.0125-19.2019}, publisher = {Society for Neuroscience}, abstract = {Operant (instrumental) conditioning is a laboratory analog for voluntary behavior and involves learning to make a response for a reinforcing outcome. The prelimbic cortex (PL), a region of the rodent medial prefrontal cortex, and the dorsomedial striatum (DMS), have been separately established as important in the acquisition of minimally-trained operant behavior. Despite dense anatomical connections between the two regions, experimenters have only recently linked projections from the PL to the posterior DMS in the acquisition of an operant response. Yet, it is still unknown if these projections mediate behavioral expression, and if more anterior regions of the DMS (aDMS), which receive dense projections from the PL, are also involved. Therefore, we utilized designer receptors exclusively activated by designer drugs (DREADDs) to test whether or not projections from the PL to the anterior DMS influence the expression of operant behavior. Rats underwent bilateral PL-targeted infusions of either a DREADD virus (AAV8-hSyn-hM4D(Gi)-mCherry) or a control virus (AAV8-hSyn-GFP). In addition, guide cannulae were implanted bilaterally in the aDMS. Rats were tested with both CNO (DREADD ligand) and vehicle infusions into the aDMS. Animals that had received the DREADD virus, but not the control virus, showed attenuated responding when they received CNO microinfusions into the aDMS, compared to vehicle infusions. Patch clamp electrophysiology verified the inhibitory effect of CNO on DREADDs-expressing PL neurons in acute brain slices. GFP-expressing control PL neurons were unaffected by CNO. The results add to the recent literature suggesting that connections between the PL and aDMS are important for the expression of minimally-trained operant responding.Significance statement Only very recently has it been shown that prelimbic cortex projections to the posterior dorsomedial striatum are important in the acquisition of operant responding. Here, we show that prelimbic cortex projections to the anterior dorsomedial striatum are important in the expression of operant responding.}, URL = {https://www.eneuro.org/content/early/2019/09/11/ENEURO.0125-19.2019}, eprint = {https://www.eneuro.org/content/early/2019/09/11/ENEURO.0125-19.2019.full.pdf}, journal = {eNeuro} }