GnRH Neuron Excitability and Action Potential Properties Change with Development But Are Not Affected by Prenatal Androgen Exposure

Abstract Gonadotropin-releasing hormone (GnRH) neurons produce the final output from the brain to control pituitary gonadotropin secretion and thus regulate reproduction. Disruptions to gonadotropin secretion contribute to infertility, including polycystic ovary syndrome (PCOS) and idiopathic hypogonadotropic hypogonadism. PCOS is the leading cause of infertility in women and symptoms resembling PCOS are observed in girls at or near the time of pubertal onset, suggesting that alterations to the system likely occurred by that developmental period. Prenatally androgenized (PNA) female mice recapitulate many of the neuroendocrine phenotypes observed in PCOS, including altered time of puberty, disrupted reproductive cycles, increased circulating levels of testosterone, and altered gonadotropin secretion patterns. We tested the hypotheses that the intrinsic properties of GnRH neurons change with puberty and with PNA treatment. Whole-cell current-clamp recordings were made from GnRH neurons in brain slices from control and PNA females before puberty at three weeks of age and in adulthood to measure GnRH neuron excitability and action potential (AP) properties. GnRH neurons from adult females were more excitable and required less current to initiate action potential firing compared with three-week-old females. Further, the afterhyperpolarization (AHP) potential of the first spike was larger and its peak was delayed in adulthood. These results indicate development, not PNA, is a primary driver of changes to GnRH neuron intrinsic properties and suggest there may be developmentally-induced changes to voltage-gated ion channels in GnRH neurons that alter how these cells respond to synaptic input.


Introduction
Gonadotropin-releasing hormone (GnRH) is the final output from the brain for the neuroendocrine control of reproduction. GnRH release occurs in an episodic manner from neurons located in the midventral preoptic area and hypothalamus (Clarke and Cummins, 1982;Moenter et al., 1992;Knobil and Neill, 1994). GnRH acts on the anterior pituitary to stimulate synthesis and release of the gonadotropins follicle-stimulating hormone (FSH) and luteinizing hormone (LH); low-frequency GnRH pulses favor these processes for FSH whereas high-frequency GnRH pulses favor LH (Wildt et al., 1981;Haisenleder et al., 1991). In females, FSH and LH regulate ovarian follicle maturation and steroidogenesis. Disruptions to gonadotropin secretion patterns, implying altered GnRH release, can contribute to infertility including idiopathic hypothalamic hypogonadism (Legro, 2003;Tsutsumi and Webster, 2009;Sidhoum et al., 2014) and polycystic ovary syndrome (PCOS; Legro, 2003;Burt Solorzano et al., 2012). PCOS is the leading cause of infertility in females, affecting up to 20% of females of reproductive age according to the Rotterdam criteria, for which patients must exhibit at least two of three symptoms: hyperandrogenemia, oligo/anovulation, and/or polycystic ovarian morphology (Chang and Katz, 1999;Legro, 2003;McCartney and Marshall, 2016). Hyperandrogenemic PCOS is diagnosed in 8-10% of all women and gonadotropin release in these women is characterized by persistently high LH pulse frequency and elevated LH/FSH ratio (Taylor et al., 1997;McCartney et al., 2002;Patel et al., 2004;Tsutsumi and Webster, 2009), suggesting at least some changes are occurring centrally at the level of GnRH release.
To study underlying central mechanisms, we must turn to animal models that recapitulate aspects of PCOS (Walters et al., 2018). In several species, including primates, mice, rats and sheep, in utero exposure to androgens produces offspring that develop reproductive neuroendocrine phenotypes similar to hyperandrogenemic PCOS (Levine et al., 1985;Robinson et al., 2002;Sullivan and Moenter, 2004;Foecking et al., 2005;Mahoney and Padmanabhan, 2010;Roland and Moenter, 2011;Witham et al., 2012;Moore et al., 2015;Abbott et al., 2016;Coyle and Campbell, 2019). In adulthood, prenatally androgenized (PNA) mice exhibit disrupted estrous cycles, elevated LH pulse frequency and increased testosterone levels compared with controls (Roland and Moenter, 2011;Moore et al., 2015;Dulka and Moenter, 2017;Berg et al., 2018). GnRH neurons from adult PNA mice have a higher action potential (AP) firing rate (Roland and Moenter, 2011;Dulka and Moenter, 2017). This elevated firing rate is likely driven at least in part by PNA-induced increases in GABAergic transmission to these cells (Sullivan and Moenter, 2004;Berg et al., 2018); unlike most neurons, GABA signaling via the GABA A receptor can induce action potential firing by GnRH neurons Herbison and Moenter, 2011). Whether or not intrinsic changes in GnRH neurons also contribute to the increased firing rate of these cells in adult PNA animals is not known.
The onset of PCOS is thought to occur near puberty (Ibañez et al., 1993;Franks, 2002;Rosenfield, 2007;Ibáñez et al., 2009;Burt Solorzano et al., 2010) and studies in PNA mice suggest neurobiological changes are already occurring before puberty is complete. Specifically, in contrast to the increased firing rate observed in GnRH neurons from PNA adults, GnRH neurons from three-week-old PNA mice have reduced firing rates compared with controls (Roland and Moenter, 2011;Dulka and Moenter, 2017). Interestingly, GnRH neurons from three-week-old PNA mice still receive increased GABAergic transmission compared with controls (Berg et al., 2018). The decrease in GnRH neuron activity in combination with the increase in GABA transmission to these cells in three-week-old PNA mice was surprising because we would expect the increase in GABA drive to increase firing activity unless the response to GABA is altered. Indeed, PNA treatment decreases the percentage of GnRH neurons from threeweek-old PNA mice that increase firing in response to locally-applied GABA, suggesting their response to GABA is indeed altered. This was not attributable to changes in either baseline membrane potential or the reversal potential for GABA A -mediated current (Berg et al., 2018), indicating that either other elements of GABA A receptor signaling, for example receptor composition, and/or changes in other intrinsic properties of GnRH neurons contribute to the reduced ability of GABA to induce action potential firing in GnRH neurons from prepubertal PNA mice.
Here, we began to characterize the response of GnRH neurons to current injection to examine how development and/or PNA treatment alter intrinsic properties of these cells. Specifically, we examined the excitability of GnRH neurons, defined as the number of action potentials generated as a function of current injected, and their response to hyperpolarizing current injections. By performing these studies in both control and PNA mice at three weeks of age and in adulthood, we gained valuable information about the normal development of GnRH neuron excitability during the pubertal process, and how PNA treatment affects this developmental trajectory. We hypothesized that GnRH neurons from PNA mice are less excitable at three weeks of age compared with controls, but become more excitable than controls during adulthood.

Materials and Methods
All chemicals were acquired from Sigma-Aldrich unless noted.

Animals
GnRH-GFP (Tg(Gnrh1-EGFP)51Sumo MGI:6158457) mice (Suter et al., 2000) were bred in our colony. All mice were provided with water and Harlan 2916 (nonbreeders) or 2919 (breeders) chow ad libitum and were held on a 14/10 h light/ dark cycle with lights on at 3 A.M. Eastern Standard Time. To generate PNA mice, female GnRH-GFP transgenic mice on a C57Bl/6J background and a CD1 female were bred with a C57Bl/6J male and monitored daily for a copulatory plug (day 1 of pregnancy). The CD1 dam assists in providing maternal care and nutrition. On days 16-18 of pregnancy, GnRH-GFP dams were injected subcutaneously with 225 mg/d of dihydrotestosterone (DHT) for PNA or sesame oil for vehicle controls. Combined litter sizes were adjusted to ,15 pups by culling CD1 pups to standardize nutrition. Adult female mice were studied on the morning of diestrus, determined via vaginal cytology and uterine mass. All procedures were approved by the Institutional Animal Care and Use Committee of the University of Michigan.

Recording solutions and data acquisition
Whole-cell patch-clamp recordings in current-clamp mode were conducted to investigate the intrinsic properties of GnRH neurons from PNA and control mice before and after puberty. The pipet solution contained (in mM): 125 K gluconate, 20 KCl, 10 HEPES, 5 EGTA, 0.1 CaCl 2 , 4 MgATP, and 0.4 NaGTP, 305 mOsm, pH 7.2 with NaOH. This solution is based on the native intracellular chloride concentrations in GnRH neurons determined using gramicidin perforatedpatch recordings Berg et al., 2018). A 14.5-mV liquid junction potential was negated online before each recording. During recordings, brain slices were continuously perfused with carboxygenated ACSF (3 ml/min) and kept at 30-31°C with an inline-heating unit (Warner Instruments Model SH-27B). GFP-positive GnRH neurons were visualized with infrared differential interference contrast and fluorescence microscopy on an Olympus BX51WI microscope. All recordings were made using an EPC-10 patch-clamp amplifier and a computer running PatchMaster software (HEKA Elektronik). To monitor recording quality, input resistance, series resistance, baseline current, and capacitance were monitored throughout experiments from the averaged membrane current response to 16 hyperpolarizing voltage steps from -70 mV (5 mV, 20 ms, acquisition 100 kHz, filter 10 kHz). Data were analyzed using IgorPro (Wavemetrics). Only recordings with input resistance of .500 MV, stable compensated series resistance of ,20 MV and a stable capacitance were used for analysis.

Experimental design
GnRH neuron excitability, the number of action potentials generated as a function of current injection, and action potential properties were monitored in GnRH neurons in the preoptic area of brain slices from prepubertal (18-21 d) and adult (84-130 d) gonad-intact control and PNA female mice; adults were studied in diestrus, determined by vaginal cytology. PNA status was confirmed by monitoring age at vaginal opening (VO), estrous cyclicity by vaginal lavage for 14 consecutive days, and measuring anogenital distance (AGD) between 70 and 84 d of age (mean of three successive daily measures).

Excitability and action potential property analysis
To characterize GnRH neuron excitability and action potential properties, current-clamp recordings were obtained (20-kHz acquisition, 10-kHz filter) in the presence of ionotropic glutamate and GABA receptor antagonists [20 mM D-APV (Tocris), 10 mM CNQX (Tocris), 100 mM picrotoxin]. Bridge balance (95%) was used for all experiments. Cells were maintained within 1.5 mV of -70 mV. Basal membrane potential was calculated during the 1 ms before the start of current injection. Current steps (500 ms, 5-pA increments from 0 to 140 pA, 5-pA decrements from 0 to -50 pA) were delivered to test the membrane potential response. The first action potential (AP) observed at the rheobase, the minimum current needed to induce action potentials, was analyzed in detail. Action potential threshold was defined as the potential at which the membrane potential slope exceeded 1 V/s. Action potential latency was the time from start of the current injection to threshold. Rate of rise was the maximum voltage derivative from threshold to peak. Full width of the action potential at half maximum (FWHM) between threshold and peak, and afterhyperpolarization (AHP) time and amplitude relative to threshold were also calculated. The membrane response to hyperpolarizing current injection was quantified as the difference between the average membrane potential over 1 ms around the peak sag potential and the average membrane potential over 1 ms at the peak steady state potential. The membrane response of GnRH neurons following the termination of the hyperpolarizing current injection was quantified as the difference between the average of membrane potential for 1 ms around the peak repolarization potential and the basal membrane potential before current injection.

Statistics
Data are reported as mean 6 SEM, with individual values shown when practical. Statistical analyses were made using Prism 9 (GraphPad Software). Data were tested for normal distribution with Shapiro-Wilk. Details of specific tests are provided in the results. Statistical tables for two-way ANOVAs report the differences in means and associated 95% confidence interval (CI) defined for age (three weekadult), treatment (control-PNA), and interaction [(adult-CONadult-PNA) -(three-week CONthree-week PNA)]; a was set to 0.05 and n indicates the number of mice for Figure 1 and number of cells for electrophysiology.

Results
Verification of prenatal androgenization phenotype PNA-induced differences were confirmed in the present study; for mice in which electrophysiology was done at three weeks of age and in adulthood, these aspects were verified in littermates raised to adults as the PNA phenotype is consistent among littermates. As reported (Roland and Moenter, 2011;Dulka and Moenter, 2017;Berg et al., 2018;Gibson et al., 2021), vaginal opening (VO) in the present study occurred at a younger age (unpaired, two-tailed Student's t test; control n = 19 mice, PNA n = 16 mice) and lower body mass (twotailed Mann-Whitney U test) in PNA females (Fig. 1A,B; Table  1). Anogenital distance (AGD) was increased in adult PNA mice (unpaired, two-tailed Student's t test; Fig. 1C; Table 1; control n = 15 mice, PNA n = 17 mice) and estrous cycles were disrupted. Specifically, PNA females spent fewer days in proestrus and more days in diestrus (x 2 , Fig. 1D,E; Table 1; control n = 17 mice, PNA n = 17 mice).

Recording quality parameters and passive properties of GnRH neurons
Passive properties and series resistance were used to assess recording quality. There were no differences among these parameters between depolarizing and hyperpolarizing current injection protocols, and they were combined for quality assessment (two-way ANOVA; three-week control n = 15 cells, three-week PNA n = 18 cells, adult control n = 15 cells, adult PNA n = 17). There were no differences in compensated series resistance or capacitance among groups ( Fig. 2A,B; Table 2). Input resistance was greater and holding current was more hyperpolarized in cells from adult than three-week-old mice (Fig. 2C,D; Table 2).
GnRH neurons from adult females are more excitable than those from three-week-old females Whole-cell current-clamp recordings were used to assess excitability measured as the number of action potentials generated as a function of current injection. Figure  3A shows representative membrane potential traces (top) in response to current injection (bottom) for each group; only three steps are shown for clarity. GnRH neurons from adult mice generated more action potentials in response to depolarizing current than those from three-week-old females, but PNA treatment had no effect on excitability (three-way, repeated-measures ANOVA, Fig. 3B; Table 3; three-week control n = 14 cells, three-week PNA n = 12 cells, adult control n = 12 cells, adult PNA n = 17 cells). Based on results of the three-way ANOVA, data were consolidated by age or treatment for comparison and reanalyzed by two-way, repeated-measures ANOVA, which found a difference for age and thus was followed by Bonferroni post hoc ( Fig. 3B; Table 3). Age, but not treatment, consolidated data show that GnRH neurons from adult mice fire more action potentials in response to depolarizing current than those from three-week-old females.

Developmental stage alters GnRH neuron action potential properties
To generate hypotheses about specific ionic current changes that may underlie the above observations, we  Table 1; ***p , 0.005, ****p , 0.0001.
Research Article: New Research examined properties of the first spike generated at rheobase (Tables 4, 5). Representative traces aligned by AP threshold are shown in Figure 3C for each group. There were no differences in AP threshold, latency, rate of rise, amplitude, or FWHM among groups (two-way ANOVA; Fig. 3D-H). The rheobase was lower in cells from adult mice, consistent with the increased excitability and input resistance (Fig. 3I). Despite the similarity of the spike phase of the AP, the AHP differed among groups. Specifically, the AHP peaked later in adult compared with three-week-old females regardless of PNA treatment (Fig. 3J), and the amplitude approached the level set for significance for being larger in adults (Fig. 3K). Together, these observations suggest that changes in the action potential properties of GnRH neurons are primarily driven by development.
There are no differences in the response of GnRH neurons to hyperpolarizing current during development or with PNA treatment Representative membrane voltage traces (top) in response to the hyperpolarizing current are shown in Figure 4A; only three current steps are shown for   Table 2. clarity. No cells exhibited rebound spikes in response to termination of hyperpolarizing current injection. To assess membrane sag typically associated with activation of hyperpolarization-activated current (Ih), comparisons were made among cells that were hyperpolarized to a membrane potential between À90 and À95 mV. Negative current between À10 to À45 pA was needed to hyperpolarize GnRH neurons to this membrane potential range and less hyperpolarizing current was required to achieve this membrane potential in adulthood compared with threeweek-old females regardless of PNA treatment (Table 6; three-week control n = 11 cells, three-week PNA n = 10 cells, adult control n = 11 cells, adult PNA n = 13 cells). The membrane potential difference between peak of the sag and steady-state (sag) was both minimal and similar among experimental groups ( Fig. 4B; Table 6). There was also no difference in GnRH neuron rebound depolarization after termination of the current injection ( Fig. 4C; Table 6). These data suggest neither development nor PNA treatment alter the response of GnRH neurons to hyperpolarizing input within the range tested.

Discussion
Reproduction is centrally controlled by the release pattern of GnRH. Disruptions in GnRH release are postulated to occur in hyperandrogenemic women with PCOS based on the observed increased frequency of pulsatile LH secretion. We used a mouse model that recapitulates the neuroendocrine aspects of PCOS to test the hypotheses that intrinsic properties of GnRH neurons change during typical development, and that these developmental changes are altered by PNA treatment (Roland and Moenter, 2011;Moore et al., 2015;Dulka and Moenter, 2017;Berg et al., 2018). Data supported the first hypothesis but rejected the second as the intrinsic properties of GnRH neurons tested change with age but are not affected by PNA treatment. Specifically, GnRH neurons from adult mice are more excitable and have altered action potential properties compared with those from three-week-old mice.
During typical development, the spontaneous action potential firing rate of GnRH neurons is dynamic, with mean GnRH neuron firing rate peaking at three weeks of age before decreasing to lower levels in adulthood (Roland and Moenter, 2011;Dulka and Moenter, 2017). In contrast, the firing rate in GnRH neurons from PNA mice is steady throughout development. As a result, firing rate of GnRH neurons from PNA mice is lower than controls at three weeks of age, but increased in adulthood (Roland and Moenter, 2011;Dulka and Moenter, 2017). These observations indicate that both development and PNA treatment play a role in shaping the activity of these neurons. This overall firing rate is shaped by a combination of the intrinsic properties of and synaptic inputs to GnRH neurons, as well as interactions with non-neuronal cells like glia (Prevot and Sharif, 2022). The present work suggests that the intrinsic properties of these neurons are developmentally regulated but not affected by PNA treatment. This finding is consistent with work in which transcriptome profiling of GnRH neurons from the same groups revealed more developmental changes than changes that were induced by PNA treatment (Burger et al., 2020). Whereas GnRH neuron membrane response to depolarizing current changed between the ages studied, there were no differences among groups in membrane response to hyperpolarizing current or in rebound firing. To begin to formulate hypotheses about the changes that might underlie the observed postpubertal increase in GnRH neuron excitability, properties of the first action potential were measured. In adulthood, GnRH neurons also have increased input resistance, which likely is a strong contributor to two other changes observed with development, specifically increased excitability and reduced amount of current needed to induce firing (rheobase). Cells from adults also had a larger and delayed peak of the afterhyperpolarization potential regardless of PNA treatment. Changes to voltage-gated channels likely underlie these developmental differences. Potassium currents are major regulators of neuronal excitability and also sculpt the afterhyperpolarization potential. Blocking 4-aminopyridine-sensitive potassium channels in dorsal root ganglion neurons decreases the latency to fire in response to current  Tables 3 and 4. Research Article: New Research injection and increases firing frequency (Vydyanathan et al., 2005). In motor neurons derived from patients with amyotrophic lateral sclerosis, partial pharmacological block of voltage-gated potassium currents reduces neuronal hypoexcitability and restores typical firing patterns (Naujock et al., 2016), and in cerebellar granule cells, a transient A-type potassium current increases during development concomitant with an increase in rheobase and spike latency (Shibata et al., 2000).
Similar effects of voltage-gated potassium conductances on excitability have been observed in hypothalamic neurons regulating reproduction. Estradiol-dependent increases in the membrane response to GABA and AMPA conductances applied with dynamic clamp in arcuate kisspeptin neurons were attributable to reduced A-type potassium currents (DeFazio et al., 2019). Consistent with this, increasing the A-type potassium current in these neurons with dynamic clamp made firing irregular and increased the interval between action potentials (Mendonça et al., 2018). In GnRH neurons from adults, the A-type potassium current is reduced in a model of estradiol positive feedback (ovariectomized plus estradiol) compared with cells from ovariectomized mice (DeFazio and . This was associated with a reduced latency to action potential firing (DeFazio and  and may contribute to the increase in spontaneous firing rate observed during positive feedback (Christian et al., 2005;Silveira et al., 2017). These observations, along with the delayed AHP, increased AHP amplitude and differences in rheobase and excitability observed in the current study suggest that there may be developmental changes to potassium currents in GnRH neurons.   In addition to the voltage-gated potassium currents mentioned above, voltage-gated sodium currents are critical for the initiation and propagation of action potentials, and also regulate neuronal excitability (Armstrong and Hille, 1998;Hille, 2001). In animal models of sensory neuropathies, mutations impairing fast inactivation of voltagegated sodium channels increase excitability, hyperpolarize action potential threshold, and increase action potential FWHM (Xiao et al., 2019). In the present study, the lack of differences in these parameters, despite the differences in excitability, suggests that any changes in voltage-gated sodium channels in GnRH neurons from the groups studied are likely subtle. Voltage-gated calcium channels can also regulate neuronal excitability (Simons, 1988;Simms and Zamponi, 2014). Inhibition of T-type voltage-gated calcium channels in a subpopulation of medial habenula neurons reduces rebound burst firing and excitability (Vickstrom et al., 2020). Activation of voltage-gated calcium channel families can subsequently activate calcium-dependent potassium channels (Armstrong and Matteson, 1986;Storm, 1987), which can contribute to the shape and timing of the AHP and thus sculpt the firing activity of neurons (Sah and Faber, 2002). Calciumdependent potassium currents have also been detected in GnRH neurons from mice and guinea pigs; these can be modulated by estradiol feedback in mice, shape the AHP and contribute to both firing patterns and subthreshold oscillations in these cells (Bosch et al., 2002;Liu and Herbison, 2008;Chu et al., 2012). Direct studies of specific currents will be needed to address these questions as changes to one or more voltage-gated currents cannot be ruled out by the present study, nor can PNA-induced changes despite the lack of effect of this treatment on overall excitability as quantified.
Development and PNA treatment could have independent and interacting effects on GnRH neuron activity and thus downstream reproductive function. The developmental increases in GnRH neuron excitability observed in the present study may help drive the pubertal reawakening of the reproductive neuroendocrine system. This may indicate intrinsic properties favoring firing become more important for effective neuroendocrine output in adults, perhaps because of concomitant changes in synaptic inputs. In PNA mice, the differences in spontaneous GnRH neuron firing (Dulka and Moenter, 2017) combined with the lack of PNA effects on stimulus-induced firing observed in this study focusing on overall intrinsic properties of these cells might indicate that it is primarily mechanisms upstream of GnRH neurons that are engaged by PNA treatment to increase spontaneous firing rate of these cells in adults. In this regard, PNA treatment increases GABAergic neurotransmission to GnRH neurons at three weeks of age and in adulthood and increases appositions on GnRH neurons by GABAergic afferents originating in part from the arcuate hypothalamus (Moore et al., 2015). Increased activation of GABAergic afferents to GnRH neurons increases LH levels and LH pulse frequency (Silva et al., 2019). While LH is not a direct measure of GnRH neuron activity, pulse frequency is a good bioassay under most circumstances (Moenter, 2015). Together, these observations suggest increased GABAergic input to GnRH neurons correlates with increased GnRH neuron activity.
Other intrinsic mechanisms could contribute to altered response to GABA. First, it is possible that PNA treatment alters chloride cotransporter function. At the soma, there were no differences in the reversal potential of GABA A -receptor-mediated current between control and PNA females at three weeks of age (Berg et al., 2018). It is not known, however, whether differences exist in the chloride homeostasis along GnRH neuron projections; if there is a lower intracellular chloride along the processes, for example, this could blunt the influence of more distal inputs. Second, PNA treatment may alter the number, type or subcellular location of GABA A -receptors on GnRH neurons. One  , and GnRH neuron rebound following the hyperpolarizing current relative to baseline membrane potential (C). Statistical parameters shown in Table 5. study in adults showed that PNA increases the amplitude of GABAergic postsynaptic currents and alters their kinetics in adulthood (Sullivan and Moenter, 2004), while another study did not show these alterations through development although a trend toward higher amplitude PSCs in adults was observed (Berg et al., 2018). It is important to bear in mind that these measures are made in the soma and that changes that occur in distal cell compartments may not be detected.
It is also possible that other afferents, including glutamate and neuropeptides, are altered by PNA treatment. In this regard, neurons in the hypothalamic arcuate nucleus called KNDy neurons use glutamate, kisspeptin, neurokinin B, and dynorphin to communicate. KNDy neurons are postulated to serve as a GnRH-pulse generator (Han et al., 2015). Of note, the frequency of LH pulses in female rodents and in both male and female sheep is increased by PNA treatment (Veiga-Lopez et al., 2008;Recabarren et al., 2012;Yan et al., 2014;Silva et al., 2019). KNDy neurons express receptors for gonadal steroids (Ruka et al., 2016;Silva et al., 2019), and thus serve as an important site for steroidal feedback modulation of GnRH neuron activity and a possible site of androgen action for the PNA phenotype (Pielecka-Fortuna et al., 2011;Yeo et al., 2014;Adams et al., 2018;Nagae et al., 2021). KNDy neurons have been studied in PNA mice, and while both GABAergic and glutamatergic appositions to these cells are reduced in PNA mice (Moore et al., 2021), neither the spontaneous firing rate nor the burst firing patterns of KNDy neurons change with development or with PNA treatment (Gibson et al., 2021). KNDy-mediated changes in input to GnRH neurons could be generated by alterations in neuromodulator expression (Goodman et al., 2013;Ahn et al., 2015). How other afferents to GnRH neurons change throughout development and/or with PNA treatment and whether they shape GnRH neuron firing remains to be studied.
Here, we demonstrate that changes to overall GnRH neuron excitability and action potential properties are primarily driven by age, and not altered by prenatal androgen exposure. Future work will focus on how specific ion channels are altered and how these changes shape the response of GnRH neurons to the input they receive. These findings contribute to the overall knowledge of GnRH neurons, how their intrinsic properties are shaped during development and with PNA treatment and generate testable hypotheses as to the cause of these developmental changes.