Metabotropic Glutamate Receptor 2/3 (mGluR2/3) Activation Suppresses TRPV1 Sensitization in Mouse, But Not Human, Sensory Neurons

Abstract The use of human tissue to validate putative analgesic targets identified in rodents is a promising strategy for improving the historically poor translational record of preclinical pain research. We recently demonstrated that in mouse and human sensory neurons, agonists for metabotropic glutamate receptors 2 and 3 (mGluR2/3) reduce membrane hyperexcitability produced by the inflammatory mediator prostaglandin E2 (PGE2). Previous rodent studies indicate that mGluR2/3 can also reduce peripheral sensitization by suppressing inflammation-induced sensitization of TRPV1. Whether this observation similarly translates to human sensory neurons has not yet been tested. We found that activation of mGluR2/3 with the agonist APDC suppressed PGE2-induced sensitization of TRPV1 in mouse, but not human, sensory neurons. We also evaluated sensory neuron expression of the gene transcripts for mGluR2 (Grm2), mGluR3 (Grm3), and TRPV1 (Trpv1). The majority of Trpv1 + mouse and human sensory neurons expressed Grm2 and/or Grm3, and in both mice and humans, Grm2 was expressed in a greater percentage of sensory neurons than Grm3. Although we demonstrated a functional difference in the modulation of TRPV1 sensitization by mGluR2/3 activation between mouse and human, there were no species differences in the gene transcript colocalization of mGluR2 or mGluR3 with TRPV1 that might explain this functional difference. Taken together with our previous work, these results suggest that mGluR2/3 activation suppresses only some aspects of human sensory neuron sensitization caused by PGE2. These differences have implications for potential healthy human voluntary studies or clinical trials evaluating the analgesic efficacy of mGluR2/3 agonists or positive allosteric modulators.


Introduction
Species differences between rodents and humans have been proposed to contribute to the low success rate of analgesic drug development (Le Bars et al., 2001;Whiteside and Kennedy, 2010;Woolf, 2010;Mao, 2012). In preclinical research, putative analgesics are often identified and exclusively evaluated in rodent tissues and/or pain models before entering clinical trials, in which such drugs seldom demonstrate efficacy (Kissin, 2010;Woolf, 2010;Moore et al., 2013). Therefore, using primary human neurons to validate preclinical rodent findings is an appealing strategy to improve the translational success of basic pain research findings. With this goal in mind, we and others have established approaches to obtain and use human sensory neurons to better understand human nociceptor physiology (Baumann et al., 1996;Anand et al., 2006;Han et al., 2015;Zhang et al., 2015;Sapio et al., 2016;Valtcheva et al., 2016;Rostock et al., 2017).
Our recent studies on cultured human dorsal root ganglia (DRG) neurons suggest peripheral mGlu2/3 receptors may be clinically relevant analgesic targets. We demonstrated both anatomical and functional expression of group II mGluRs in human DRG . Importantly, as in mice, mGluR2/3 activation blocked human nociceptor membrane hyperexcitability produced by the inflammatory mediator prostaglandin E2 (PGE 2 ), indicating that a mechanism for peripheral analgesia may be conserved across species . Rodent studies suggest that mGlu2/3 receptors expressed on sensory neuron peripheral terminals can also reduce sensory neuron sensitization by suppressing sensitization of TRPV1 (Yang and Gereau, 2002;Du et al., 2008;Carlton et al., 2009Carlton et al., , 2011, a nonselective cation channel that detects noxious stimuli and is critical for inflammationinduced peripheral sensitization (Caterina et al., 1997(Caterina et al., , 2000Davis et al., 2000;Moriyama et al., 2005). The present study tested whether the same mechanism is conserved in humans. We used sensory neurons obtained from organ donors without chronic pain to determine whether mGluR2/3 activation blocks inflammation-induced sensitization of TRPV1 in human neurons. We demonstrate that group II mGluR activation suppresses PGE 2induced sensitization of TRPV1 calcium responses in mouse, but not human, sensory neurons. Interestingly, this functional difference was not explained by species differences in coexpression of the TRPV1 gene transcript with mGlu2 or mGlu3 receptor gene transcripts.

Animals
All experiments were performed in compliance with protocols approved by the Animal Studies Committee of Washington University in St. Louis (Protocol nos. 20150246 and 20160097). Experiments were conducted on 5-8-wkold C57BL/6J male and female mice (Jackson Laboratory, RRID:IMSR_JAX000664). Mice were housed in an animal facility with a 12-h light-dark cycle and given food and water ad libitum.

Donors
Human tissue was obtained in compliance with procedures approved by Mid-America Transplant (St. Louis, MO), and the Human Research Protection Office at Washington University in St. Louis provided an International Review Board waiver. Human DRG were obtained from organ donors with full legal consent for use of tissue for research. Only donors without a history of chronic pain were used in this study (Table 1).

Human DRG cultures
Human DRG from the first through fifth lumbar vertebrae were surgically extracted and cultured as described in detail previously (Valtcheva et al., 2016). Briefly, after extraction, fat and dura were trimmed away from the ganglia. DRG were minced, incubated in papain for 1 h, rinsed, and incubated in collagenase for 1 h. Both enzyme solutions were made up in an N-methyl-D-glucamine artificial cerebrospinal fluid solution (Sigma-Aldrich). DRG were manually triturated with fire-polished Pasteur pipettes to dissociate neurons, passed through a 100-m filter (VWR), and plated onto poly-D-lysine/collagen-coated 12-mm glass coverslips. Neurons were maintained in culture for up to 9 d in the medium described above (mean time in culture: 6 d). Every 3 d, half of the culture medium was replaced with fresh media to ensure neuronal health.

Calcium imaging
Calcium imaging experiments were performed on mouse and human sensory neurons on days in vitro (DIV) 2 and 3-9, respectively. Acutely after culturing human DRG neurons, satellite glial encased neurons, and thus accurate physiology experiments could not be performed until the glial peeled off and exposed the neuron plasma membrane, which occurred after 3-4 DIV as reported previously (Valtcheva et al., 2016). Mouse calcium imaging experiments were therefore initially performed on DIV 4. Strikingly, we found that only 2% of mouse DRG neurons responded to 100 nM capsaicin on DIV 4, which we interpreted as a functional downregulation of TRPV1. Therefore, we chose to perform mouse calcium imaging experiments on DIV 2 such that recordings were not performed acutely after culturing neurons, yet were completed before TRPV1 functional downregulation.
Cultured neurons from mice and humans were incubated with 3 g/mL of the ratiometric calcium indicator Fura-2 AM (Life Technologies) for 45 min. Neurons were then incubated in external solution for 15 min to allow for de-esterification of Fura-2 AM. External solution consisted of (in mM): 130 NaCl, 5 KCl, 2 CaCl 2 , 1 MgCl 2 , 30 glucose, and 10 Hepes. For recordings, coverslips were placed into a chamber and perfused with room temperature external solution. Cells were viewed under an inverted microscope (Olympus Optical), and fluorescent images were acquired every 2 s using a Hamamatsu ORCA camera (Hamamatsu). SimplePCI Software (HCImage, Hamamatsu) was used to identify regions of interest surrounding Fura-2 AM-loaded neurons a priori and to record fluorescence emission at alternating excitation wavelengths of 357 and 380 nm.
The experimental protocol entailed a 2-min baseline in external solution followed by a 20-s bath application of 100 nM capsaicin (Sigma-Aldrich), a 3-min wash with external solution, then a treatment condition entailing application of either 7 min of vehicle (external solution), 6 min of 1 M prostaglandin E2 (PGE 2 , Tocris), or 1 min of 10 M (2R, 4R)-4-aminopyrrolidine-2,4-dicarboxylate (APDC, Tocris) alone followed by 6 min of 10 M APDC plus 1 M PGE 2 . Immediately after treatment, a second pulse of 100 nM capsaicin was bath-applied, neurons were washed for 6 min with external solution, and a 10-s pulse of 50 mM KCl was applied to test for cell viability. At least 2 treatment conditions were tested for a given mouse or donor tissue preparation. All drugs were diluted in external solution and bath-applied at a rate of 2 mL/min. Stock solutions of 2.8 mM PGE 2 and 10 mM APDC were made up in DMSO and water, respectively. Peak calcium responses were calculated by dividing the absolute increase in Fura-2 AM signal after stimulus application by the proceeding 30-s baseline Fura-2 AM signal. The response threshold to capsaicin was defined as an increase of Ն10% from baseline signal. Cells that did not respond to high KCl were excluded from calcium imaging analysis.

Fluorescent in situ hybridization (RNAscope)
At the conclusion of mouse and human calcium imaging experiments, neurons were fixed on ice with 4% paraformaldehyde/4% sucrose for 15 min and washed with PBS. Fluorescent in situ hybridization (FISH) studies were performed according to the protocol for cultured adherent cells using the RNAscope Multiplex Fluorescent Assay (Advanced Cell Diagnostics) with minor modifications. After dehydration and rehydration of cells in ethanol, glass coverslips were mounted onto glass slides using ethyl cyanoacrylate. Neurons were treated with protease III diluted 1:10 (mouse) or 1:5-7.5 (human) at room temperature for 10 min. Species-specific target probes for Trpv1, Grm2, and Grm3 (Table 2) were combined, applied to neurons, and allowed to hybridize for 2 h at 40°C in a humidified oven. A series of incubations were then performed to amplify hybridized probe signal and label target probes with the assigned fluorescence detection channel (C1-C3). Coverslips were counterstained with DAPI using ProLong Gold Antifade Mountant (Invitrogen). Neurons were imaged at 40ϫ using a Leica SPE confocal microscope (Leica Microsystems). Fields of interest were identified in the DAPI channel. Fiji (Image J, NIH, RRID: SCR: 002285) software was used to calculate neuron diameter and manually quantify single RNA molecule signals. In the RNAscope assay, each punctate dot represents a single target RNA molecule. However, to reduce the likelihood of false positives, mouse and human neurons were defined as positive for a given RNA target if they had Ն4 or Ն2 puncta, respectively, based on the range in neuron puncta density observed for each species. Although we used the same mouse and human tissues used in both assays (Table 1), neuron populations analyzed using FISH were not identical to those analyzed via calcium imaging.

Statistical analyses
The experimenter was blind to treatment condition and gene of interest throughout analysis of calcium imaging and in situ hybridization data, respectively. Microsoft Excel, GraphPad Prism, R, and the R package Ime4 (RRID: SCR_015654) were used for data organization and statistical analyses (Bates et al., 2014;Team, 2017). Calcium imaging data were analyzed using (1) unpaired t tests and Bonferroni correction for multiple comparisons and (2) linear mixed-effects model (LMM) analyses. LMM analyses controlled for donor as a random effect and donor sex, donor age, and DIV as fixed effects. As the primary objective of the present study was to make species comparisons, we were not sufficiently powered to evaluate the effects of mouse age or sex on calcium imaging outcomes. Species comparisons of the percentage of capsaicin-responsive neurons and gene transcript expression were made using 2 tests and Bonferroni correction for multiple comparisons, when appropriate. When describing and discussing species differences in gene transcript expression, we default to mouse mRNA nomenclature. Superscript letters listed with p-values correspond to the statistical tests shown in Table 3.

mGluR2/3 suppress PGE 2 -induced TRPV1 sensitization in mouse, but not human, sensory neurons
TRPV1 is sensitized by the cAMP/PKA pathway, which is stimulated by inflammatory mediators such as PGE 2 (Lopshire and Nicol, 1998;De Petrocellis et al., 2001;Bhave et al., 2002;Mohapatra and Nau, 2003;Meves, 2006). In contrast, group II mGlu receptor activation inhibits adenylyl cyclase and subsequent cAMP production (Conn and Pin, 1997;Johnson and Schoepp, 2008). To determine whether mGluR2/3 activation blocks TRPV1 sensitization, we quantified capsaicin-induced calcium responses of mouse and human sensory neurons. Two 20-s pulses of 100 nM capsaicin were bath-applied to DRG neurons, and the degree of TRPV1 sensitization was defined as the response ratio of the peak of the second capsaicin response divided by the peak of the first cap-   (Fig. 1A, B). In mouse sensory neurons, bath application of PGE 2 between capsaicin pulses significantly increased the capsaicin response ratio compared with vehicle (Fig. 1A, C a ). Coapplication of the selective group II mGluR agonist APDC with PGE 2 significantly reduced the response ratio compared with PGE 2 alone (Fig. 1A, C b ). As an additional measure of TRPV1 sensitization, we quantified the total calcium load, or area under the curve (AUC) of a subset of capsaicin . Experiments concluded with a pulse of 50 mM KCl to determine cell viability. The degree of TRPV1 sensitization is expressed as a response ratio calculated by dividing the peak amplitude of Cap 2 by the peak amplitude of Cap 1 (A, dashed lines). C, In mouse DRG neurons, PGE 2 significantly increased the capsaicin response ratio compared with vehicle ‫ءءءء(‬ p ϭ 2.1 ϫ 10 -5 ; n ϭ 143-150 neurons, n ϭ 4 preps/condition). Coapplication of APDC with PGE 2 blocked this effect and significantly reduced the response ratio compared to PGE 2 alone ‫ءء(‬ p ϭ 0.0081; n ϭ 89 -150 neurons, n ϭ 3-4 preps/condition). D, PGE 2 also significantly increased the capsaicin response ratio of human DRG neurons compared with vehicle ‫ءءءء(‬ p ϭ 3.0 ϫ 10 -6 ; n ϭ 59 -71 neurons, n ϭ 5-6 donors/condition); whereas coapplication of APDC did not suppress PGE 2 -induced increases in the capsaicin response ratio (p ϭ 1, n ϭ 59 -64 neurons, n ϭ 6 donors/condition), which remained significantly greater than vehicle ‫ءء(‬ p ϭ 0.0053, n ϭ 64 -71 neurons, n ϭ 5-6 donors/condition). Capsaicin response ratios were compared using unpaired t tests and a Bonferroni correction for multiple comparisons. Data are presented as mean Ϯ SEM. E, Compared with those of mice, a greater percentage of human DRG neurons responded to 100 nM capsaicin ( 2 ϭ 15.45, ‫ءءءء‬ p ϭ 8.5 ϫ 10 -5 , mouse: 405/1761 neurons, n ϭ 4 preps, human: 223/731 neurons, n ϭ 8 donors). F, LMM regression correction for impact of individual donor, as well as donor age and sex, did not alter human capsaicin response ratio analysis statistical outcomes compared with t tests alone.
Negative Results responses. Consistent with the effects on capsaicin peak response ratios, application of PGE 2 significantly increased AUC compared with vehicle, and coapplication of APDC with PGE 2 significantly reduced this effect (data not shown). These findings confirm our previously published work in cultured sensory neurons obtained from CD-1 mice in which we also demonstrated that suppression of PGE 2 -induced TRPV1 sensitization by APDC is blocked by the group II mGluR antagonist LY341495, and thus is attributable to mGlu2/3 receptor activation (Yang and Gereau, 2002).
In human sensory neurons, we found that although application of PGE 2 between capsaicin pulses significantly increased the capsaicin response ratio compared with vehicle, the response ratio after coapplication of APDC with PGE 2 did not significantly differ from application of PGE 2 alone (Fig. 1B, D c,d ). We observed similar results for total calcium load of capsaicin responses (data not shown). Unlike rodents used in preclinical studies, human organ donors display wide demographic and genetic diversity. To correct for between-donor heterogeneity, we performed a LMM regression. As the effect of age and sex on human sensory neuron physiology is of broad interest to pain researchers, these parameters were included as covariates in our LMM regression. Interestingly, we found that correcting for impact of individual donor, as well as donor age and sex, did not alter our capsaicin response ratio outcome (Fig. 1F). Moreover, LMM regression analysis indicated that the day in vitro at which calcium imaging experiments were performed did not influence capsaicin response ratios for any of the conditions tested (t ϭ 0.105, p ϭ 0.9182 e ). Taken together, these results demonstrate that mGluR2/3 activation suppresses PGE 2 -induced TRPV1 sensitization in mouse, but not human, sensory neurons.
We were also interested in whether the same percentage of mouse and human sensory neurons respond to capsaicin. A neuron was defined as capsaicin responsive if it responded to either the first or second pulse of capsaicin. Notably, compared with vehicle conditions, application of PGE 2 did not increase the number of neurons that did not respond to the first pulse of capsaicin but did respond to the second pulse of capsaicin in either species. Therefore, data were pooled across treatment conditions for calculations of percentage of capsaicinresponsive neurons. Of the total number of sensory neurons evaluated in calcium imaging studies, 23.0% of mouse sensory neurons versus 30.5% of human sensory neurons responded to 100 nM capsaicin, indicating that human sensory neurons are modestly more capsaicin responsive (Fig. 1E f ). These results are consistent with our initial observations that human sensory neurons exhibit greater chemosensitivity to algogens and pruritogens compared with rodents . In the present study, we chose to use 100 nM capsaicin to test for modulation of TRPV1 desensitization. Previous studies using higher concentrations of capsaicin (200 nM to 1 M) indicate that capsaicin elicits calcium responses in a greater proportion of mouse DRG and trigeminal neurons, ranging from 30% to 70% (Davis et al., 2000;Elitt et al., 2008;Barabas and Stucky, 2013;Ren et al., 2014;Valtcheva et al., 2015;Mohammed et al., 2017).

Mouse and human sensory neurons share similar Trpv1, Grm2, and Grm3 expression and coexpression profiles
We hypothesized that the observed species differences in mGluR2/3 functional modulation of TRPV1 could be due to reduced coexpression between TRPV1 and mGlu2 and/or mGlu3 receptors in human versus mouse sensory neurons. We previously demonstrated mGluR2 immunoreactivity in human sensory neurons . However, because of the lack of highly selective mGluR3 antibodies suitable for immunohistochemistry (García-Bea et al., 2016), we assessed expression of TRPV1, mGluR2, and mGluR3 mRNA transcripts (referred to as Trpv1, Grm2, and Grm3, respectively) in dissociated sensory neurons using RNAscope FISH. The mouse DRG neurons evaluated in FISH studies ranged from 10.0 to 35.5 m in diameter, with a mean diameter of 17.7 Ϯ 0.2 m for the total population and 20.3 Ϯ 0.4 m for Trpv1 ϩ neurons ( Fig. 2A). In comparison, human DRG neurons ranged from 10.0 to 56.3 m in diameter, with a mean diameter of 31.9 Ϯ 0.5 m for the total population and 33.9 Ϯ 0.9 m for TRPV1 ϩ neurons (Fig. 2B). The mean diameter of the total human DRG neuron population was significantly larger than that of mice (Fig. 2C g ). Our findings closely resemble the size distribution of mouse DRG neurons reported previously (Dirajlal et al., 2003;Barabas et al., 2012;O'Brien et al., 2015). Previous human studies show that the average sensory neuron diameter in unfixed tissues is between ϳ40 and 60 m Xu et al., 2015;Han et al., 2016;Zhang et al., 2017), further highlighting the species difference in sensory neuron size.
With respect to mRNA expression, of the total population of mouse DRG neurons, 37.2% of neurons were positive for the Trpv1 gene transcript, 61.5% were positive for Grm2, and 30.9% were positive for Grm3 (Fig. 2D). Similar transcript expression was observed in human sensory neurons, with 32.2% of all neurons positive for TRPV1, 52.7% positive for GRM2, and 29.5% positive for GRM3 (Fig. 2E). Notably, a significantly greater percentage of total DRG neurons expressed Grm2 compared to Grm3 in both mice ( 2 ϭ 74.56, p ϭ 1.0 ϫ 10 -15 h ) and humans ( 2 ϭ 28.83, p ϭ 8.0 ϫ 10 -8 i ). Although there was a trend toward an increased percentage of Grm2 ϩ neurons in mouse compared with human DRG ( 2 ϭ 2.229 with Bonferroni correction, p ϭ 0.077 j ), there were no significant species differences in the expression of the transcripts of interest.
Analysis of transcript coexpression showed that the majority of Trpv1 ϩ mouse DRG neurons coexpressed Grm2, Grm3, or both. To elaborate, 33.3% of Trpv1 ϩ neurons coexpressed Grm2, 7.5% coexpressed Grm3, and 44.2% coexpressed both Grm2 and Grm3 (Fig. 2F). Of TRPV1 ϩ human DRG neurons, 41.0% coexpressed GRM2, 8.4% coexpressed GRM3, and 32.5% coexpressed both GRM2 and GRM3 (Fig. 2G). No significant species differences were observed in the coexpression of Trpv1 with Grm2 and/or Grm3 gene transcripts. Thus, these findings suggest that the absence of mGluR2/3-induced suppression of TRPV1 sensitization in human DRG neurons cannot be explained by reduced coexpression of TRPV1 with group II mGluRs at the gene transcript level.

Discussion
Peripheral targets for pain relief are greatly desired given the centrally mediated side effects, including addiction and misuse of current frontline analgesics such as opioids. In both mice and humans, activation of group II mGluRs blocks sensory neuron membrane hyperexcitability elicited by the inflammatory mediator PGE 2 . In the present study, we demonstrate that suppression of inflammation-induced TRPV1 sensitization represents an additional mechanism by which mGluR2/3 reduce peripheral sensitization in mouse, but not human, sensory neurons. We further show equivalent colocalization of Trpv1 with Grm2 and Grm3 mRNA tran-scripts in mouse and human DRG neurons, suggesting that disparities in coexpression do not explain species differences in the functional modulation of TRPV1 by group II mGluRs. These findings indicate that although mGluR2/3 activation decreases sensory neuron sensitization in both mice and humans, mechanisms of peripheral analgesia are not fully conserved across species.

mGluR2/3 functional differences in mouse and human sensory neurons
That the mGlu2/3 receptor agonist APDC did not suppress PGE 2 -induced TRPV1 sensitization in human DRG neurons was a surprising observation. Foremost, cDNA and amino acid sequences of human and rodent mGluR2 and mGluR3 display at least 90% homology (Flor et al., 1995;Makoff et al., 1996;Johnson and Schoepp, 2008). In turn, APDC exhibits comparable potency at rodent and human group II mGluRs with respect to inhibition of stimulated cAMP responses (Schoepp et al., 1995). Our recent  147 neurons). B, Size distribution of total and TRPV1 ϩ human DRG neuron populations; mean diameter of total neurons: 31.9 Ϯ 0.5 m (n ϭ 258 neurons, n ϭ 5 donors), mean diameter of TRPV1 ϩ neurons: 33.9 Ϯ 0.9 m (n ϭ 83 neurons). Data are reported as mean Ϯ SEM. C, The mean diameter of total human DRG neurons was significantly larger than that of total mouse DRG neurons (unpaired t test, ‫ءءءء‬ p ϭ 1.0 ϫ 10 -14 ). Data are presented as mean Ϯ SD. Percentage of total mouse (D) and human (E) DRG neurons that expressed Trpv1, Grm2, and Grm3, as well as the percentage of neurons that coexpressed one mRNA transcript with another. Pie charts showing the percentage of Trpv1 ϩ mouse (F) and human (G) DRG neurons that coexpressed Grm2, Grm3, or both transcripts. No significant differences in gene transcript expression of total neurons or Trpv1 ϩ neuron subpopulations were observed between species.
Negative Results finding that APDC decreases excitability and increases action potential threshold in PGE 2 -treated sensory neurons of both species further suggests mGluR2/3 functional homology in mouse and human sensory neurons . Existing behavioral and in vitro rodent studies strongly suggest that inhibition of cAMPdependent TRPV1 sensitization is another mechanism by which mGluR2/3 can block sensory neuron sensitization (Yang and Gereau, 2002;Du et al., 2008;Carlton et al., 2009Carlton et al., , 2011. Here, we substantiate these findings by demonstrating that APDC blocks PGE 2 -induced TRPV1 sensitization in C57BL/6J mice. However, this observation did not translate in human sensory neurons despite the apparent similarities of mGluR2/3 function between species. There are multiple potential explanations for the lack of translation of mGluR2/3 functional modulation of TRPV1 from mice to humans. For example, in addition to PKA, PGE 2 -induced intracellular signaling cascades can activate other kinases known to sensitize TRPV1, including PKC and c-Src kinase (Vellani et al., 2001;Numazaki et al., 2002;Jin et al., 2004;Moriyama et al., 2005). Thus, although we observed PGE 2 -induced sensitization of capsaicin responses in both mouse and human sensory neurons, it is possible that in contrast to mice, PGE 2 -induced TRPV1 sensitization in humans occurs via a predominantly PKA-independent pathway that is not influenced by mGlu2/3 receptor activation. Further investigation of the intracellular mechanisms that underlie PGE 2 -induced TRPV1 sensitization in human sensory neurons is therefore needed.
Another important consideration is that although we observed equivalent coexpression of Grm2 and Grm3 gene transcripts in Trpv1 ϩ mouse and human DRG neurons, whether equivalent coexpression of TRPV1 and mGluR2/3 also extends to the protein level remains unclear. For instance, it is possible that species differences exist in the regulation of translation, posttranslational modifications, and subcellular compartmentalization of TRPV1 and mGlu2/3 receptors. TRPV1 immunoreactivity has been demonstrated in human DRG neurons, peripheral nerves, and intra-epidermal nerve fibers (Anand et al., , 2015Facer et al., 2007;Li et al., 2015;Han et al., 2016). However, until selective mGluR2 and mGluR3 antibodies suitable for immunohistochemistry are generated, our ability to evaluate the coexpression and subcellular localization of group II mGluRs with TRPV1 remains limited. Importantly, although mGlu2/3 receptor activation does not modulate sensitization of TRPV1 in humans, the expression of GRM2 and/or GRM3 in the majority of small-diameter (Ͻ50 m) TRPV1 ϩ human DRG neurons suggests that these receptors are well positioned to modulate nociceptor activity by alternative mechanisms. Thus, mGluR2/3 remain putative human peripheral analgesic targets.

Sensory neuron expression of Grm2, Grm3, and Trpv1
Existing immunohistochemical analyses of rodent DRG neurons demonstrate high colocalization of group II mGlu receptors with TRPV1, with 93% of TRPV1-positive neurons expressing mGluR2/3 and effectively all mGluR2/3-positive neurons expressing TRPV1 (Carlton et al., 2009). Here we demonstrate that the majority (Ն81%) of Trpv1 ϩ mouse and human sensory neurons also express Grm2 and Grm3 gene transcripts. In contrast, we found that in both mice and humans, only a subset (45%-62%) of either Grm2 ϩ or Grm3 ϩ neurons also expressed the Trpv1 transcript. Importantly, prior immunohistochemistry studies using nonselective mGluR2/3 antibodies and RNAsequencing analysis of homogenized DRG precluded analyses of which group II mGlu receptor is predominantly expressed in sensory neurons and to what extent mGluR2 and mGluR3 are coexpressed within the same neurons. We show for the first time that Grm2 is more highly expressed than Grm3 in mouse and human sensory neurons. Further, while Grm2 and Grm3 are coexpressed in a subset (38%-77%) of mouse and human sensory neurons, the transcripts are also expressed individually. These findings suggest that mGluR2 may play a larger role in modulating nociceptor excitability than mGluR3. Indeed, previous behavioral studies demonstrate that the analgesic efficacy of group II mGlu receptor agonists persists in mGluR3 -/-, but not mGluR2 -/-, mice, suggesting a greater role for mGluR2 than mGluR3 in pain regulation (Zammataro et al., 2011). As repeated dosing with group II mGlu receptor agonists causes analgesic tolerance in rodents (Jones et al., 2005;Chiechio et al., 2009), alternative strategies to reinforce endogenous activation of mGluR2 may be required to effectively target group II mGlu receptors for clinical pain relief. Our results suggest that recently developed mGluR2-specific positive allosteric modulators may be promising agents for blockade of peripheral sensitization (Galici et al., 2006;Asseri et al., 2015).

Distinctions between human and rodent sensory neurons
In addition to differences in mGlu2/3 receptor function, we demonstrate that human sensory neurons possess distinct fundamental properties including increased diameter and an increased percentage of capsaicin-responsive neurons compared with those of mice. Using calcium imaging, we found that 30.5% of human DRG neurons responded to 100 nM capsaicin. However, it is possible that a larger proportion of human sensory neurons expresses TRPV1 and/or responds to capsaicin. For instance, previous immunohistochemical analysis of human DRG showed that roughly 55% of all DRG neurons were TRPV1-immunoreactive . Further, in calcium imaging experiments, ϳ60% of ganglionectomized DRG neurons removed due to chronic intractable pain responded to 100 nM capsaicin (Baumann et al., 1996(Baumann et al., , 2004. These findings suggest that an even greater species difference in capsaicin-responsive neurons may exist between mice and humans than we report here. Of course, variability in experimental conditions or donor demographics, genetic diversity, and pain-related health conditions could underlie differences in TRPV1 expression across human studies. For this reason, developing an extensive donor tissue bank to investigate DRG neuron gene and protein expression as well as to further characterize sensory neuron subpopulations will allow for more complete comparisons between species, and perhaps more interestingly, among donor subpopulations. Nevertheless, while only a small number of comparative studies of rodent and human sensory neurons have been conducted, it is becoming increasingly clear that species differences exist in gene expression, ion channel properties, and action potential firing patterns (Baldo et al., 2013;Han et al., 2015;Chang et al., 2017;Ray et al., 2017;Rostock et al., 2017;Zhang et al., 2017). Therefore, human sensory neurons represent a vital tool for improving our understanding of human nociceptor physiology under both normal and pathologic conditions. Further, using human sensory neurons to assess the validity of putative analgesic targets identified in rodents may lead to increased translational success of preclinical findings.