Dynamic Foraging Behavior Performance Is Not Affected by Scn2a Haploinsufficiency

Abstract Dysfunction in the gene SCN2A, which encodes the voltage-gated sodium channel Nav1.2, is strongly associated with neurodevelopmental disorders including autism spectrum disorder and intellectual disability (ASD/ID). This dysfunction typically manifests in these disorders as a haploinsufficiency, where loss of one copy of a gene cannot be compensated for by the other allele. Scn2a haploinsufficiency affects a range of cells and circuits across the brain, including associative neocortical circuits that are important for cognitive flexibility and decision-making behaviors. Here, we tested whether Scn2a haploinsufficiency has any effect on a dynamic foraging task that engages such circuits. Scn2a+/− mice and wild-type (WT) littermates were trained on a choice behavior where the probability of reward between two options varied dynamically across trials and where the location of the high reward underwent uncued reversals. Despite impairments in Scn2a-related neuronal excitability, we found that both male and female Scn2a+/− mice performed these tasks as well as wild-type littermates, with no behavioral difference across genotypes in learning or performance parameters. Varying the number of trials between reversals or probabilities of receiving reward did not result in an observable behavioral difference, either. These data suggest that, despite heterozygous loss of Scn2a, mice can perform relatively complex foraging tasks that make use of higher-order neuronal circuits.


Introduction
Deleterious mutations in the gene SCN2A, which encodes the voltage-gated sodium channel Na v 1.2, constitute one of the leading risk factors for neurodevelopmental disorders, increasing the odds of developing autism spectrum disorder and intellectual disability (ASD/ID) to levels beyond those calculable from studies of large cohorts (e.g., an infinite odds-ratio; Sanders et al., 2012;Ben-Shalom et al., 2017;Satterstrom et al., 2020;Fu et al., 2022;Rolland et al., 2023).ASD-associated SCN2A dysfunction is typically a result of haploinsufficiency, where loss of only one gene copy cannot be compensated for by the remaining allele (Wolff et al., 2017;Ben-Shalom et al., 2017;Sanders et al., 2018;Begemann et al., 2019).Scn2a is expressed throughout the brain (Kang et al., 2004;GTEx Consortium, 2015).In neocortex, Scn2a is expressed predominantly on the plasma membranes of pyramidal cells (Hu et al., 2009;Li et al., 2014;Yamagata et al., 2017), and previous work has shown that Scn2a haploinsufficiency in mice results in reduced dendritic excitability in prefrontal cortical pyramidal cells (Spratt et al., 2019;Nelson et al., 2022;Tamura et al., 2022).Beyond neocortex, Scn2a is also expressed in striatal medium spiny neurons (Miyazaki et al., 2014), where a .50%reduction in expression can result in neuronal hyperexcitability (Zhang et al., 2021), as well as in midbrain dopamine neurons (Yang et al., 2019), where its functional role has not yet been elucidated.This expression pattern suggests that Scn2a haploinsufficiency might affect behaviors involving corticostriatal circuits and their modulation via midbrain dopaminergic sources.
There are other behavioral assays to measure behavioral flexibility, however.Dynamic foraging, for example, requires the animal to use prior outcomes to guide current decisions, as in the water maze, but also requires learning in an uncertain environment.Choices are not associated with specific outcomes.Rather, rewards are delivered probabilistically, and the reward probabilities can switch over time (Soltani and Izquierdo, 2019).Because the task is nontrivial and challenging, it can be applied to nonhuman primates (Donahue and Lee, 2015) and humans (Sutton and Barto, 2018) and is thus more readily translatable.Several animal ASD models had impaired reversal learning in dynamic foraging tasks, although the nature of their errors varied (Roh et al., 2020;Alvarez et al., 2023;Schmitt et al., 2023).Numerous studies have shed light onto the neural circuits involved in dynamic foraging, implicating regions including medial frontal cortex (Atilgan et al., 2022), orbitofrontal cortex (Groman et al., 2019), and dorsal striatum (Tai et al., 2012).More specifically, silencing PFC projections to dorsomedial striatum, including those originating in layer 5, increased choice bias (and thus reduced flexible choice) in two similar foraging tasks (Nakayama et al., 2018;Bari et al., 2019).Given the importance of Na v 1.2 for dendritic integration in PFC layer 5 pyramidal cells (Spratt et al., 2019;Nelson and Bender, 2021) and its broad expression in additional circuits that support behavioral flexibility and foraging behavior, we sought to determine whether Scn2a haploinsufficiency had an effect on a dynamic foraging task.
Here, we show that Scn2a 1/À mice do not exhibit behavioral differences compared with WT littermates, and both groups can readily learn the task.For both deterministic and probabilistic reward contingencies, mice of both genotypes were able to adapt their choice behavior equally after a reward contingency reversal.Scn2a 1/À mice and WT littermates did not show differences in motivation as measured by self-initiated intertrial interval and also appeared to use similar strategies to obtain reward.Furthermore, varying block length or reward probabilities did not unmask a behavioral difference in Scn2a 1/À animals.There were no differences across sexes for any of the behavioral metrics analyzed.As a final note, we observed spontaneous seizures in four Scn2a 1/À animals, which may relate to housing conditions required to motivate animals to perform these behaviors.Overall, these data demonstrate that mice can perform tasks that require learning and behavioral flexibility in light of heterozygous loss of Na V 1.2 expression.

Animals
All animal procedures were performed in accordance with the University of California, San Francisco and Yale University Institutional Animal Care and Use Committees.Postnatal day (P)54-P119 Scn2a 1/À mice or wild-type (WT) littermates of both sexes on the C57BL/6J background were used.Scn2a 1/À mice were originally described by Planells-Cases et al. (2000).Animals were genotyped with PCR.Mice were maintained on a 12/12 h light/dark cycle and had ad libitum access to food.No animals were excluded based on behavioral performance.Two of 50 animals failed to complete the entire behavioral assay.One WT mouse died during the training period for unknown reasons.One Scn2a 1/À mouse experienced a terminal seizure immediately after a training session when returned to their home cage.The experimenter was blind to the animal genotypes while running the behavior.

Freely moving behavior
All experiments were conducted in purpose-built acrylic boxes (roughly 599 Â 799, left and right poke walls were angled to form a pentagon).Each box had three custombuilt pokes, each with a white LED and infrared emitters and receivers (Sparkfun).The left and right pokes dispensed a solution of 10% sucrose in water through metal tubes fitted into the poke, and the sucrose delivery was controlled with solenoid pinch valves (NResearch).The task was run using an mbed microcontroller and custom MATLAB and Statescript (Spike Gadgets) scripts, and all behavioral analyses were completed with custom MATLAB scripts.
Three days before the start of behavioral training, animals were placed on water deprivation for 24 h and on subsequent water restriction to maintain weight at 85% predeprivation weight.If insufficient sucrose solution was consumed during the behavioral session, supplemental water was given in the home cage to maintain weight.
In the first stage of training, animals learned to receive reward from the side ports by poking their noses in the central nosepoke.On days 1 and 2, animals were placed in the behavioral boxes, and self-initiated pokes into the center poke triggered automatic reward delivery (;3 ml 10% sucrose solution) in the left (for one session) or right (for the other session, counterbalanced across animals) port.Once the animal poked its nose into the baited port, additional reward was dispensed.Animals completed 100 trials or 1.5 h of training, whichever came first, each day.
In the second stage of training, animals learned to collect reward from both side ports in a single session.Animals self-initiated trials by poking their noses into the center nosepoke.A light would turn on at the center nosepoke to signal reward availability.Reward availability was set at 100% for one side port and 0% for the other, and animals could choose between the two.A nose poke to the high reward port resulted in immediate reward delivery (;2 ml 10% sucrose solution) at that port.The location of the high reward probability port underwent uncued reversals every 100 trials or once the animal selected the high reward port 9 of the 10 previous trials.Animals performed an average of 714 trials a day and were trained on this version of the task until they reached 80% performance 2 d in a row or after 10 sessions.
In the full version of the foraging task, animals self-initiated trials by poking their noses into the center nosepoke.Reward availability for each port was independently assigned to 60% for one port (high reward probability) versus 15% for the other (low reward probability) unless noted in the text and figures.Once reward was assigned to a port, the reward was available until the animal chose that port (Sugrue et al., 2004;Lau and Glimcher, 2008;Fonseca et al., 2015;Bari et al., 2019).The location of the high reward probability port underwent uncued reversals every 80 trials unless noted in the text and figures.Animals performed an average of 938 trials per session on this full version of the task.

Head-fixed behavior
Before surgery, the mouse was given carprofen (5 mg/ kg, s.c.; 024751, Butler Animal Health) and dexamethasone (3 mg/kg, intramuscular; 002458, Henry Schein Medical Animal Health).Anesthesia was induced with 3% isoflurane in oxygen, and lowered to 1-1.5% during surgery.The mouse was positioned in a stereotaxic apparatus (940, David Kopf Instruments) and sat on a 38°C water-circulating heating pad (Stryker Corp).The scalp was removed to expose the skull.A custom-made stainless steel headplate (eMachineShop) was glued onto the skull with C&B Metabond (Parkell).After the surgery, carprofen (5 mg/kg, s.c.) was injected each day for the following 3 d.The mouse recovered for at least 7 d after the surgery before the start of the behavioral training.
A comprehensive guide on constructing the apparatus is available at https://github.com/Kwan-Lab/behavioralrigs.For the behavioral apparatus, a specialized lick port with two lick spouts, made from blunted 20-gauge stainless-steel needles, was positioned in front of the mouse.For controlled fluid delivery at the lick spouts, two solenoid fluid valves (MB202-V-A-3-0-L-204, Gems Sensors & Controls) were employed.Each spout's water delivery could be independently regulated.The quantity of water dispensed per pulse was tuned to ;4 ml by adjusting the duration of the electrical pulse administered to each valve via a second data acquisition unit (USB-201, Measurement Computing).To produce the auditory cue, a pair of speakers (S120, Logitech) was positioned in front of the mouse.For head fixation, the head plate was securely held in place using a stainless-steel holder (eMachineShop).The mouse sat within an acrylic tube (8486K433; McMaster-Carr), allowing for minor postural adjustments while restricting major movements.Contact between the tongue and the lick spouts were detected using a battery-powered electronic circuit.Signals from this circuit were transmitted to a computer via a data acquisition unit (USB-201, Measurement Computing).The captured data were logged using the Presentation software (Neurobehavioral Systems).The entire apparatus was enclosed within an audiovisual cart, the walls of which were insulated with soundproof acoustic foams (5692T49, McMaster-Carr).
Mice were fluid-restricted during behavioral training.On training days, the animal received all of its water intake from behavioral training that occurred one session per day, 6 d per week.On nontraining days and on days if its weight fell below 85% of their pretraining value, water was provided ad libitum in their home cage for 5 min.Before the behavioral training, the animal was handled and habituated to head fixation for increasing durations over 3 d.The training procedure involves four phases including three phases to shape the behavior before the final task phase.
During phase 0 (;2 d), the experimenter manually administered 50 water rewards through each port (100 rewards in total), with the goal to elicit reliable licks.In cases where the animal did not readily engage in licking to consume the water rewards, the experimenter used a blunted syringe to gently guide the animal toward the spout, facilitating a licking response.
During phase 1 (;1 d), the animal was trained to alternate between the two lick ports to receive water rewards.At the beginning of each trial, an auditory cue (5 kHz, 0.2s-long pure tone) was played.Subsequent to cue onset, there was a 5s response window for the animal to act.The first lick within the response window is the animal's response.The playback of the auditory cue was terminated early if a response was recorded before the entire stimulus was played.The animal was trained to alternate its choices.Specifically, if the mouse received a reward on the left side on the current trial, it must choose the right side on the next trial to trigger the next reward, and vice versa.The session would end if the animal did not lick during the response window for 20 consecutive trials.
During phase 2 (;2 d), the animal was trained to alternate, while also having to suppress licking before the go cue.Phase 2 is the same as phase 1, with two modifications.First, the response window was shortened to 2 s.Second, a no-lick period was introduced between trials.The no-lick period began 3 s after the animal's response.Initially, the duration of the no-lick period was determined by drawing a random number from a truncated exponential distribution (l ¼ 0.3333, minimum ¼ 1, maximum ¼ 5).If any lick was detected during the no-lick period, an additional duration drawn from the same truncated exponential distribution would be added to the duration of the no-lick period.This iterative addition could be repeated for a maximum of five times.Therefore, the entire duration of the no-lick period could range between 1 and 25 s and depended on the animal's ability to successfully suppress its licking.
Phase three is the two-armed bandit task.The trial timing is the same as phase 2, including the no-lick period before the go cue.Trials were organized in blocks with each block having a different set of reward probabilities.In a 70:10 block, the left lick spout carried a 70% likelihood of delivering water if chosen and the right lick port carried a 10% likelihood of delivering water if chosen.Conversely, in a 10:70 block, the reward probabilities for the left and right ports were 10% and 70%, respectively.At the beginning of each session, the block type (70:10 or 10:70) was randomly chosen.An uncued transition in block type occurred when the mouse chose the higher value side in 10 trials and then performed an additional random number of trials (L Random ) with value drawn from a truncated geometric distribution (m ¼ 11, minimum ¼ 0, maximum ¼ 30).The session would terminate when the animal did not respond for 20 consecutive trials.Animals were deemed to be experts if they chose the side with higher reward probability for at least 50% of the trials over three consecutive sessions.We analyzed the data from sessions after animals reached expert performance.

Statistics
All data are displayed as means 6 SE or as box plots (medians, quartiles, and 90% tails) with individual points overlaid.Sample sizes were chosen based on standards in the field.No assumptions were made for data distribution, and the specific statistical tests used and relevant values are noted in the figure legends.Significant level was set for an a level of 0.05, and multiple comparisons corrections were used when appropriate.Statistical analyses were performed using the Real Statistic Pack plugin for Microsoft Excel (Release 8.0).

Results
Scn2a 1/2 mice learn the foraging task similar to WT littermates To test behavioral flexibility and learning dynamics in conditions of Scn2a haploinsufficiency, we implemented a dynamic foraging task that has been shown to involve corticostriatal circuits and midbrain dopamine signaling (Samejima et al., 2005;Kennerley et al., 2006;Ragozzino, 2007;Lau and Glimcher, 2008;Rutledge et al., 2009;Hamid et al., 2016;Del Arco et al., 2017;Ueda et al., 2017;Donahue et al., 2018;Nakayama et al., 2018;Bari et al., 2019;Cox et al., 2023).Briefly, water deprived and freely moving mice learned to self-initiate trials in the center nosepoke and subsequently to choose between two reward ports with differing reward probabilities (Fig. 1A).Animals acquired the behavioral task over a 12d training period, the majority of which was spent on a version of the task where reward probabilities on the two ports were fixed at 0% and 100%.The location of the high reward probability port underwent uncued reversals after 100 trials or after the animal selected the high reward port 9 of the 10 previous trials (Fig. 1B).Animals performed this version of the task for 10 trials or until they reached 80% performance on two adjacent sessions (criterion).
We first asked whether Scn2a 1/À mice took longer to learn the behavior than their wild-type (WT) littermates.For each training day and animal, we calculated the fraction of trials where the animal chose the high reward port.Scn2a 1/À and WT mice did not differ in their learning curves, and there was no difference across sexes (Fig. 1C).Since both genotypes selected the high reward side equally across sessions, we next asked whether the two genotypes adapted behaviors differently after reward contingencies reversed.For each animal, we calculated the probability that the animal chose the high reward side on trials after a block change.Scn2a 1/À and WT mice did not show differences in their behavioral adaption on the first day of training (Fig. 1D).
Once animals were well-trained on the task (the day animals reached criterion or day 10), there was also no difference in Scn2a 1/À animals' ability to reverse after a block reversal (Fig. 1E; note that animals perform the task very well once they reach criterion and often experienced block reversals after 10 trials).If anything, heterozygous mice tended to pick the higher reward side slightly more frequently later in the block (Fig. 1E).Overall, there was also no difference in performance across sex on days 1 and 10, although male Scn2a 1/À mice were slightly more likely to pick the higher reward size mid-block than their WT littermates (Fig. 1D,E).These data suggest that Scn2a 1/À mice do not have any deficits in their ability to learn the rules of a foraging task or to adapt their behavior when reward contingencies change.
Scn2a 1/2 mice perform the foraging task as well as WT littermates During training described above, reward contingencies were fixed at 0% and 100%.These reward contingencies taught the animals to sample both ports and to adapt their behavior across block changes, but did not encourage the animals to dynamically sample both reward ports within a given block.We therefore wanted to test the Scn2a 1/À mice on a version of the behavior where the chance of getting a reward is probabilistic on both sides for every trial and where animals would have to rely on their recent choice and outcome histories to guide future choices.To achieve this, we used the dynamic version of the behavior where the reward availability for each port was independently assigned to 60% for one port (high reward probability) and 15% for the other (low reward

Behavior training timeline Days 1 and 2
One side only   probability; Fig. 2A-C).Once a reward was assigned to a port, the reward was available until the animal chose that port (Sugrue et al., 2004;Lau and Glimcher, 2008;Fonseca et al., 2015;Bari et al., 2019).The location of the high reward probability port underwent uncued reversal to the opposite port every 80 trials.Animals were tested on the dynamic foraging task after reaching criterion in the training task (Figs.1B, 2A).The fraction of choices the animals made to a side "matched" the reward probability for that side, similar to what was noted in other studies (Sugrue et al., 2004;Lau and Glimcher, 2008;Fonseca et al., 2015;Tsutsui et al., 2016;Bari et al., 2019; Fig. 2C).
We first asked whether Scn2a 1/À mice were less flexible in adapting their behavior after a block change under this more unpredictable reward paradigm relative to WT littermates.However, both Scn2a 1/À mice and WT littermates flexibly changed their choice behavior after a block change and began preferring the higher rewarded port (Fig. 2D).Across the overall population, there was no difference across genotype, although in males, there was a statistically significant difference across genotype largely driven by mid-block performance (Fig. 2D).
Since there appeared to be no difference in behavioral flexibility across genotypes, we next asked whether there might be a difference in the intertrial interval (ITI), as this metric can serve as a proxy for motivational state (A.Y.Wang et al., 2013;Hamid et al., 2016;Cox et al., 2023).Because individual trials are self-initiated, we defined ITI to be the time from which the animal exited a side port to when it next entered the center nosepoke.We analyzed trials in which the animal previously received a reward and trials in which it did not separately, as we hypothesized that there may be a difference in motivational state based on previous reward as shown in other studies (Cox et al., 2023).In both the overall population and among males, WT and Scn2a 1/À mice took longer to initiate a new trial after receiving a reward, but there was no ITI difference between Scn2a 1/À mice and WT littermates in either previously rewarded or unrewarded conditions or across sexes (Fig. 2E).
Finally, we assessed whether Scn2a 1/À mice and WT littermates might be using different strategies to optimize reward.One possible strategy is win-stay, lose-switch, in which animals repeat their choice if they just received a reward or pick the opposite choice if they did not (Evenden and Robbins, 1984;Sugrue et al., 2004;Lau and Glimcher, 2008;Donahue et al., 2018).We analyzed the fraction of trials in which Scn2a 1/À mice and WT littermates chose the same port after receiving a reward ("winstay") or changed their choice after receiving no reward ("lose-switch").Both Scn2a 1/À mice and WT littermates tended to revisit the same port after receiving reward and switched ports ,50% of the time after a "lose" trial, but there was no difference across genotypes or sex (Fig. 2F).
In a separate set of experiments, we tested an independent sample of Scn2a 1/À mice and WT littermates on a similar, head-fixed version of this task (Extended Data Fig. 2-1A).Animals of both genotypes acquired and performed the task (Extended Data Fig. 2-1B,C).As with the freely moving version of the behavior, there was no difference across genotype in successfully switching sides after a block reversal (Extended Data Fig. 2-1D) or in winstay, lose-switch strategies (Extended Data Fig. 2-1E).Of note, the head-fixed assays were done in a different lab than those with freely-moving animals.Taken together, the freely-moving and head-fixed behavioral results suggest that Scn2a 1/À mice and WT littermates perform comparably and use similar strategies in this dynamic foraging task, although the head-fixed results should be considered preliminary given the lower number of animals assayed herein.
Varying block length did not affect Scn2a 1/2 mouse performance We initially tested a block length of 80 trials for the dynamic foraging task, as this was long enough to provide sufficient trials for mice to learn new reward contingencies while still ensuring that animals can adapt their behavior without developing side biases or alternate strategies.However, we wondered whether changing the block length might unmask a behavioral difference across genotypes.Therefore, we ran a subset of mice on a version of the dynamic foraging task using a block length of 40 (Fig. 3A) or 100 trials (Fig. 3B).No differences between Scn2a 1/À and WT mice were observed across any behavior, though we note that the male cohort was under-powered for the 100block experiment.
Varying reward contingencies did not affect Scn2a 1/2 mouse performance Scn2a 1/À mice and WT littermates did not appear to perform differently in the dynamic foraging task with block lengths of 40, 80, and 100 trials and reward contingencies of 15% and 60%.As a final experiment, we tested whether varying the reward contingencies within a given experiment might make it more difficult for mice to flexibly adapt their choice behavior.In a subset of behavioral sessions, we tested mice on a version of the dynamic foraging task where reward contingencies varied between 30%:30%, 15%:45%, and 10%:50% in each block of 80 trials (Fig. 4A).In another subset of sessions, we tested the same groups of mice on a version of the task where reward contingencies varied between 50%:50%, 15%:85%, and 25%:75% (Fig. 4B).We chose these reward contingencies as some were easily distinguishable (e.g., 15%:85%), some forced chance performance (e.g., 50%:50%), and some fell into an intermediate zone that is more difficult to discern (e.g., 15%:45%).Across these different reward contingencies, Scn2a 1/À mice and WT littermates were both able to adapt to the different reward contingencies (Fig. 4A,B).We did not test 0%:100% reward here, since well-trained animals did not show a behavioral difference with this reward contingency during training (Fig. 1E).Overall, our data suggest that Scn2a 1/À haploinsufficiency does not affect animals' performance on a cognitively challenging dynamic decisionmaking behavioral task.
Scn2a 1/2 mice with seizures did not perform differently on behavioral tasks In humans, SCN2A loss-of-function (LoF) is most commonly associated with ASD/ID.An estimated 20-30% of .D, Probability of choosing the new 60% baited port after the reward contingencies were reversed across all animals (left), males (center), and females (right).Scn2a 1/À mice in cyan and WT littermates in black.Bars are means 6 SEM.All animals: p ¼ 0.543 for genotype and trial; males: p ¼ 0.046 for genotype and p ¼ 0.682 for genotype and trial; females: p ¼ 0.680 for genotype and trial, repeated measures two-way ANOVA.E, Intertrial interval (ITI), measured as the time from when the animal left a side port to when it next entered the center poke, broken down by genotype and previous trial outcome for all animals (left), males (center), and females (right).Circles are individual animals.All WT, previously rewarded: median 1.8 s, interquartile range (IQR) 1.4-2.4s; all Scn2a 1/À , previously rewarded: median 1.5 s, IQR 1.2-2.1 s; all WT, previously unrewarded: median 1.1 s, IQR 0.7-1.4s; all Scn2a 1/À , previously unrewarded: median 1.0 s, IQR 0.7-1.3s; male WT, previously rewarded: median 1.7 s, IQR 1.3-2.0s; male Scn2a 1/À , previously rewarded: median 1.5 s, IQR 1.2-1.8s; male WT, children with SCN2A LoF experience seizures, usually with an onset after the first postnatal year (Sanders et al., 2018;Brunklaus et al., 2022).While other groups have reported spontaneous "absence-like" seizures in Scn2a 1/À animals (Ogiwara et al., 2018), this does not phenocopy what is observed in human patients, and we have not observed spontaneous seizure-related phenotypes in our normally housed cohort of Scn2a 1/À mice of a similar age as those studied here (Tamura et al., 2022).Here, we observed spontaneous behavioral seizures associated with this study in four of 25 Scn2a 1/À mice (from several litters and breeding pairs, three males and one female, noted in different months; none observed in WT littermates).Of note, animals studied here were housed differently than the rest of our colony, with water consumption restricted in the home cage to increase motivation for 10% sucrose solution reward during behavior.All seizures were observed at the end of a day's training when animals were being returned to their home cage.The four mice that exhibited seizures did not consume a different level of sucrose compared with animals that did not experience seizures (1.7 6 0.12 ml of solution for four mice with seizure; 1.5 6 0.14 ml for 11 Scn2a 1/À mice without seizure tested on same days, p ¼ 0.2 Mann-Whitney U test).For one male animal, the seizure progressed from spontaneous convulsions to tonic clonus  Varying block length did not affect Scn2a 1/À mouse performance.A, Left, Plot of reward contingencies for a session with a block length of 40 and 15%/60% reward probabilities.Right, Probability of choosing the new 60% baited port after the reward contingencies were reversed across all animals, males, and females.Bars are means 6 SEM.All animals: p ¼ 0.644 for genotype and trial; males: p ¼ 0.997 for genotype and trial; females: p ¼ 0.638 for genotype and trial, repeated measures two-way ANOVA.B, Left, Plot of reward contingencies for a session with a block length of 100 and 15%/60% reward probabilities.Right, Probability of choosing the new 60% baited port after the reward contingencies were reversed across all animals, males, and females.Scn2a 1/À mice in cyan and WT littermates in black.Bars are means 6 SEM.All animals: p ¼ 0.157 for genotype and trial; males: p ¼ 0.573 for genotype and trial; females: p ¼ 0.213 for genotype and trial, repeated measures two-way ANOVA.
with hindlimb extension, followed by death.This occurred during the initial training, and the animal was therefore excluded from all analyses.For the three other animals (two male, one female), seizures were noted on two separate days each.All three animals exhibited spontaneous convulsions, "popcorning" behavior, with characteristic jumping within the cage, and myoclonic jerks with loss of balance.
The two males also had seizures that progressed to tonic clonus with hindlimb extension.All three mice were tested on the entire behavior, and we did not exclude them from our analyses.We looked to see, however, whether seizures might have affected the behavioral performance of these three Scn2a 1/À mice.Their behavioral performance, measured by how quickly they adapted their behavior after a block reversal, was no different from other animals on either day 1 or the last day of training (Fig. 5).These animals were from two cohorts and thus were tested on different variations of the dynamic foraging task.They did not behave differently than other mice in any variation.As an example, all three were tested on the 40-trial block version of the dynamic foraging task (Fig. 3A) but showed no obvious behavioral differences (Fig. 5D).These data suggest that under certain conditions, seizures can be observed in Scn2a haploinsufficient animals but that they do not affect learning or performance of this foraging task.

Discussion
The voltage-gated sodium channel Na v 1.2 is expressed in medial prefrontal cortex (mPFC) as well as striatum and the axons of midbrain dopamine neurons (Miyazaki et al., 2014;Spratt et al., 2019;Yang et al., 2019;Zhang et al., 2021).Previous work has demonstrated that corticostriatal activity and dopaminergic signaling contribute to choice behaviors including in dynamic foraging tasks (Hamid et al., 2016;Bari et al., 2019;Cox et al., 2023).We therefore hypothesized that Scn2a haploinsufficiency may affect how animals learn or perform a decision-making behavior.Here, we show that Scn2a 1/À mice exhibit no differences in learning and performing a dynamic foraging behavior, even under increased task demands (varied reward contingencies and block lengths).

Reduced Scn2a expression across relevant brain regions did not translate into a behavioral phenotype
Since dysfunction in SCN2A is highly associated with autism spectrum disorder and intellectual disability (ASD/ ID; Sanders et al., 2012;Ben-Shalom et al., 2017;   Satterstrom et al., 2020;Fu et al., 2022), substantial effort has been put toward understanding the cellular and behavioral changes linked to Scn2a haploinsufficiency.Given that children with SCN2A loss-of-function (LoF) variants have notable behavioral impairments (Sanders et al., 2018) and given Na v 1.2's contribution to dendritic and axonal excitability (Bender and Trussell, 2012;Kole and Stuart, 2012;Spratt et al., 2019), it is somewhat surprising that more overt learning deficits have not been observed in Scn2a heterozygous mice.In one study, Scn2a 1/À mice took longer to learn an H-maze task (Middleton et al., 2018).Previously, it was shown that found that male, but not female, Scn2a 1/À mice were a little slower to learn the reversed contingencies in a water T-maze (Spratt et al., 2019).Scn2a 1/À mice were ultimately able to reach wildtype (WT)-level performance in both tasks.This is consistent with our findings that observed neither a learning nor performance difference.Other studies have found that Scn2a haploinsufficiency resulted in modest differences in various behavioral assays, including the open field, elevated plus maze, resident-intruder task, and fear conditioning (Shin et al., 2019;Tatsukawa et al., 2019).However, these effects were not replicated consistently across studies (Shin et al., 2019;Spratt et al., 2019;Zhang et al., 2021).Given this, it remains a challenge to connect robust cellular deficits observed in cases of Scn2a loss to behavioral readouts in heterozygotes that can be observed reliably across research groups.These difficulties are mirrored by similar issues across multiple mouse models of ASD-associated genes, especially when studied in differing strains (Silverman et al., 2022;Tabbaa et al., 2023).For studies of Scn2a, one potential solution would be to leverage conditional knock-out or gene-trap approaches to reduce Scn2a expression by .50% in circuits of interest (Eaton et al., 2021;Spratt et al., 2021).In gene-trap Scn2a-deficient mouse models, where Scn2a expression is ;25% that of WT animals, overt alterations in several behaviors and sleep dynamics were observed (Eaton et al., 2021;Ma et al., 2022).
Beyond Scn2a dosage, one could also focus more on more reflexive behaviors in mouse, as circuitry underlying such behaviors is often highly conserved across species.For example, our lab found recently that oculomotor reflexes are altered robustly by Scn2a haploinsufficiency (C.Wang et al., 2023).These reflexes are controlled in part by cerebellar circuits that are evolutionarily ancient and present in all vertebrates.Thus, conserved function of Na V 1.2 in cerebellar circuits and their involvement in behaviors that are not routinely considered in studies of ASD models (Silverman et al., 2010) may nevertheless offer high face validity across species.
Lastly, there is a final consideration of how mouse behavior relates to human behavior, and whether face validity in core ASD/ID-related behaviors (e.g., social interaction, communication, learning) could be captured appropriately in mouse models (Silverman et al., 2022).Towards this end, other animal models may be more appropriate for different traits.For example, rats can learn and perform more complex behavioral tasks than mice, potentially allowing for study of aspects of learning more closely related to those engaged by children (Wong et al., 2020;Fernandes et al., 2021;Harris et al., 2021;Anstey et al., 2022).Furthermore, other rodent species, like prairie voles, form highly complex social networks and enduring relationships that persist throughout life, allowing for study of social interaction and attachment phenotypes that are not innate to mouse (Young et al., 2002;McGraw and Young, 2010).Lastly, nonhuman primate models heterozygous for ASD-associated genes can better recapitulate ASD-associated behaviors in conditions where mouse models fail (Jiang and Ehlers, 2013;Jennings et al., 2016;Zhou et al., 2019).Overall, this suggests that, depending on the behavior in question, leveraging different animal models may be warranted.Figure 5. Scn2a 1/À mice with seizures did not perform differently on behavioral tasks.A, Same as Figure 1C.Probability of choosing the 100% baited port across training sessions across all animals.N ¼ 3 Scn2a 1/À mice (2 males, 1 female) with observed home cage seizures overlaid in purple (animals are included in N ¼ 24 mean data).The days where individual animals experienced seizures are denoted with symbols.B, Same as Figure 1D.Probability of choosing the new 100% baited port after the reward contingences were reversed on day 1 of the training block across all animals.N ¼ 3 Scn2a 1/À mice with observed home cage seizures overlaid in purple (animals are included in N ¼ 24 mean data).C, Same as Figure 1E.Probability of choosing the new 100% baited port after the reward contingences were reversed on the final training day for each mouse across all animals.N ¼ 3 Scn2a 1/À mice with observed home cage seizures overlaid in purple (animals are included in N ¼ 24 mean data).D, Same as Figure 3A.Probability of choosing the new 60% baited port after the reward contingencies were reversed (block length of 40 trials) across all animals.N ¼ 3 Scn2a 1/À mice with observed home cage seizures overlaid in purple (animals are included in N ¼ 12 mean data).

Potential effect of housing conditions on seizure susceptibility
An estimated 20-30% of children with loss-of-function SCN2A variants develop epilepsy, often after the first year of life (Sanders et al., 2018).Excess spike-and-wave discharge activity has been noted in some, but not all recordings from Scn2a 1/À mice (Ogiwara et al., 2018;Tamura et al., 2022).To our knowledge, observations here are the first of spontaneous, "popcorning" behavioral seizures in Scn2a 1/À animals, with 16% of our Scn2a 1/À cohort exhibiting behavioral seizures.This suggests that conditions used to motivate behavior, including water restriction and sucrose reward consumption, may increase seizure susceptibility in Scn2a haploinsufficient conditions.The seizures that we observed all occurred after the animals were returned to their homecage after a behavioral training session with sucrose reward, and we speculate that changes in hydration or glucose levels could contribute to seizure susceptibility, as has been reported previously (Gibbs, 1939;Andrew, 1991;Schwechter et al., 2003;Reid et al., 2011).Increasingly, there is an appreciation for potential metabolic alterations underlying epilepsies in channelopathies (Neal et al., 2023).These data suggest that Scn2a 1/À mice are more susceptible to seizures related to fluid/energy homeostasis.Future studies could focus on external stressors such as fever or fasting to better understand mechanisms that may result in seizures in Scn2a haploinsufficiency.Importantly, however, the three animals that survived multiple seizures successfully learned and performed the behavioral task (Fig. 5).These observations suggest that water restriction and/or increased sucrose consumption may increase the likelihood of seizures in these animals, but also that, even with seizures, Scn2a 1/À mice can learn the dynamic foraging behavior and adapt their choice behavior.

Figure 1 .
Figure 1.Scn2a 1/À mice learn the foraging task as well as WT littermates.A, Schematic of the behavioral box.Animals self-initiate trials in the center nosepoke and subsequently choose between the left and right port for reward.B, Timeline of behavioral training.On days 1 and 2, animals learn to receive reward from only the left or right port, 100 trials per day.Day 3 onward, animals learn to receive reward from both the left and right ports.Reward probabilities across ports are 0% and 100%, and the reward contingencies switch after 100 trials or after 9/10 correct choices.Animals train for 10 d or until they complete two sessions .80%correct, whichever comes first.After training, animals are put on the full behavioral task (details in Materials and Methods).Pink denotes the section used for analysis (in C-E, day 1 corresponds to day 3 of the training timeline).C, Probability of choosing the 100% baited port across training sessions across all animals (left), males (center), or females (right).Scn2a 1/À mice in cyan and WT littermates in black.Bars are means 6 SEM.All animals: p ¼ 0.382 for genotype and day; males: p ¼ 0.379 for genotype and day; females: p ¼ 0.451 for genotype and day, repeated measures two-way ANOVA.D, Probability of choosing the new 100% baited port after the reward contingences were reversed on day 1 of the training block across all animals (left), males (center), and females (right).Scn2a 1/À mice in cyan and WT littermates in black.Bars are means 6 SEM.All animals: p ¼ 0.950 for genotype and trial; males: p ¼ 0.950 for genotype and trial; females: p ¼ 0.630 for genotype and trial, repeated measures twoway ANOVA.E, Probability of choosing the new 100% baited port after the reward contingences were reversed on the final training day for each mouse across all animals (left), males (center), and females (right).Note that only 10 trials are plotted here as animals are well-trained and experience uncued block changes after fewer trials than on day 1.Scn2a 1/À mice in cyan and WT littermates in black.Bars are means 6 SEM.All animals: p ¼ 0.002 for genotype and trial; males: p ¼ 0.014 for genotype and trial; females: p ¼ 0.140 for genotype and trial, repeated measures two-way ANOVA.

FFigure 2 .
Figure 2. Scn2a 1/À mice perform the foraging task as well as WT littermates.A, Timeline of the behavioral training.Pink denotes section used for analysis.B, Schematic of the behavioral box.In this task, ports are set to 15% or 60% reward probabilities.C, Choice behavior from an example animal (green, 10-trial moving average) in response to changes in reward contingencies [black, ratio of left to right side reward probabilities; e.g., 60/(15 1 60) or 15/(15 1 60)].D, Probability of choosing the new 60% baited port after the reward contingencies were reversed across all animals (left), males (center), and females (right).Scn2a 1/À mice in cyan and WT littermates in black.Bars are means 6 SEM.All animals: p ¼ 0.543 for genotype and trial; males: p ¼ 0.046 for genotype and p ¼ 0.682 for genotype and trial; females: p ¼ 0.680 for genotype and trial, repeated measures two-way ANOVA.E, Intertrial interval (ITI), measured as the time from when the animal left a side port to when it next entered the center poke, broken down by genotype and previous trial outcome for all animals (left), males (center), and females (right).Circles are individual animals.All WT, previously rewarded: median 1.8 s, interquartile range (IQR) 1.4-2.4s; all Scn2a 1/À , previously rewarded: median 1.5 s, IQR 1.2-2.1 s; all WT, previously unrewarded: median 1.1 s, IQR 0.7-1.4s; all Scn2a 1/À , previously unrewarded: median 1.0 s, IQR 0.7-1.3s; male WT, previously rewarded: median 1.7 s, IQR 1.3-2.0s; male Scn2a 1/À , previously rewarded: median 1.5 s, IQR 1.2-1.8s; male WT, Figure3.Varying block length did not affect Scn2a 1/À mouse performance.A, Left, Plot of reward contingencies for a session with a block length of 40 and 15%/60% reward probabilities.Right, Probability of choosing the new 60% baited port after the reward contingencies were reversed across all animals, males, and females.Bars are means 6 SEM.All animals: p ¼ 0.644 for genotype and trial; males: p ¼ 0.997 for genotype and trial; females: p ¼ 0.638 for genotype and trial, repeated measures two-way ANOVA.B, Left, Plot of reward contingencies for a session with a block length of 100 and 15%/60% reward probabilities.Right, Probability of choosing the new 60% baited port after the reward contingencies were reversed across all animals, males, and females.Scn2a 1/À mice in cyan and WT littermates in black.Bars are means 6 SEM.All animals: p ¼ 0.157 for genotype and trial; males: p ¼ 0.573 for genotype and trial; females: p ¼ 0.213 for genotype and trial, repeated measures two-way ANOVA.
Probability of choosing the new 100% baited port after the reward contingences were reversed on day 1 of the training block across all animals (left), males (center), and females (right).Scn2a 1/À mice in cyan and WT littermates in black.Bars are means 6 SEM.All animals: p ¼ 0.950 for genotype and trial; males: p ¼ 0.950 for genotype and trial; females: p ¼ 0.630 for genotype and trial, repeated measures twoway ANOVA.E, Probability of choosing the new 100% baited port after the reward contingences were reversed on the final training day for each mouse across all animals (left), males (center), and females (right).Note that only 10 trials are plotted here as animals are well-trained and experience uncued block changes after fewer trials than on day 1.Scn2a 1/À mice in cyan and WT littermates in black.Bars are means 6 SEM.All animals: p ¼ 0.002 for genotype and trial; males: p ¼ 0.014 for genotype and trial; females: p ¼ 0.140 for genotype and trial, repeated measures two-way ANOVA.
). Scn2a 1/À mice in cyan and WT littermates in black.Bars are means 6 SEM.All animals: p ¼ 0.382 for genotype and day; males: p ¼ 0.379 for genotype and day; females: p ¼ 0.451 for genotype and day, repeated measures two-way ANOVA.D, 1/À mouse performance.A, Left, Plot of reward contingencies for a session with a block length of 80 and 30% versus 30%, 15% versus 45%, and 10% versus 50% reward probabilities.Right, Probability of choosing the high reward port (when applicable) for the 10% versus 50%, 15% versus 45%, and 30% versus 30% contingencies across all animals.Scn2a 1/À mice in cyan and WT littermates in black.Bars are means 6 SEM 10% versus 50%: p ¼ 0.351 for genotype and trial; 15% versus 45%: p ¼ 0.689 for genotype and trial; 30% versus 30%: p ¼ 0.150 for genotype and trial, repeated measures two-way ANOVA.B, Left, Plot of reward contingencies for a session with a block length of