Abstract
Persistent neuronal firing is often observed in working memory and temporal association tasks both in humans and animals, and is believed to retain necessary information in these tasks. We have reported that hippocampal CA1 pyramidal cells are able to support persistent firing through intrinsic mechanisms in the presence of cholinergic agonists. However, it still remains largely unknown how persistent firing is affected by the development of animals and aging. Using in vitro patch-clamp recordings from CA1 pyramidal cells in rat brain slices, we first show that the cellular excitability of these aged rats was significantly lower than the young rats, responding with fewer spikes to current injection. In addition, we found age-dependent modulations of input resistance, membrane capacitance, and spike width. However, persistent firing in aged (~2 years old) rats was as strong as that in young animals, and the properties of persistent firing were very similar among different age groups. In addition, spike after-hyperpolarization potential (AHP), was not increased by aging and did not correlate with the strength of persistent firing. Lastly, we estimated the depolarization current induced by the cholinergic activation. This current was proportional to the increased membrane capacitance of the aged group and was inversely correlated with the intrinsic excitability of cells. These observations indicate that robust persistent firing can be maintained in aged rats despite reduced excitability, due to the increased amount of cholinergically induced positive current.
Significant statement:
In an aging society, it is crucial to understand neural mechanisms underlying age-dependent cognitive impairments. In recent years, the importance of intrinsic cellular properties in cognitive functions such as memory has increasingly been recognized. However, research examining age-dependent alteration of intrinsic cellular mechanisms of persistent firing, which is believed to support working memory function, has so far been very scarce. In this study, we demonstrate that the ability to support persistent firing is kept intact in neurons from old rats, despite changes in other properties such as intrinsic excitability. These results identify the ability to support persistent firing as one potential cellular mechanism of rescued cognitive functions under cholinergic enhancement used in Alzheimer's disease and other dementias.
- afterhyperpolarization
- aged rats
- cholinergic agonist
- depolarization current
- hippocampus
- persistent firing
Footnotes
The authors declare no competing financial interests.
This work was supported by the International Graduate School of Neuroscience at Ruhr University Bochum, the Wilhelm und Günter Esser Foundation (B.K.), Rektorat Program at Ruhr University Bochum (M.Y.) and Deutsche Forschungsgemeinschaft Projects YO177/4-1 and YO177/4-3 (to M.Y.).
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
