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Research ArticleResearch Article: New Research, Development

Using SuperClomeleon to Measure Changes in Intracellular Chloride during Development and after Early Life Stress

Lotte J. Herstel, Carlijn Peerboom, Sten Uijtewaal, Dunya Selemangel, Henk Karst and Corette J. Wierenga
eNeuro 7 December 2022, 9 (6) ENEURO.0416-22.2022; https://doi.org/10.1523/ENEURO.0416-22.2022
Lotte J. Herstel
1Cell Biology, Neurobiology and Biophysics, Biology Department, Utrecht University, 3584 CH, Utrecht, The Netherlands
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Carlijn Peerboom
1Cell Biology, Neurobiology and Biophysics, Biology Department, Utrecht University, 3584 CH, Utrecht, The Netherlands
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Sten Uijtewaal
1Cell Biology, Neurobiology and Biophysics, Biology Department, Utrecht University, 3584 CH, Utrecht, The Netherlands
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Dunya Selemangel
1Cell Biology, Neurobiology and Biophysics, Biology Department, Utrecht University, 3584 CH, Utrecht, The Netherlands
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Henk Karst
2Utrecht Brain Center, University Medical Center Utrecht, 3584 CX, Utrecht, The Netherlands
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Corette J. Wierenga
1Cell Biology, Neurobiology and Biophysics, Biology Department, Utrecht University, 3584 CH, Utrecht, The Netherlands
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  • ORCID record for Corette J. Wierenga
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0416-22.2022
PubMed 
36635254
Published By 
Society for Neuroscience
History 
  • Received October 5, 2022
  • Revision received November 25, 2022
  • Accepted November 30, 2022
  • Published online December 7, 2022.
Copyright & Usage 
Copyright © 2022 Herstel et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Lotte J. Herstel1,*,
  2. Carlijn Peerboom1,*,
  3. Sten Uijtewaal1,
  4. Dunya Selemangel1,
  5. Henk Karst2 and
  6. Corette J. Wierenga1
  1. 1Cell Biology, Neurobiology and Biophysics, Biology Department, Utrecht University, 3584 CH, Utrecht, The Netherlands
  2. 2Utrecht Brain Center, University Medical Center Utrecht, 3584 CX, Utrecht, The Netherlands
  1. Correspondence should be addressed to C. J. Wierenga at corette.wierenga{at}donders.ru.nl.
  • C. J. Wierenga’s present address: Faculty of Science and Donders Institute, Radboud University, Nijmegen, The Netherlands.

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Author contributions

  1. Author contributions: L.J.H., C.P., and C.J.W. designed research; L.J.H. and C.P. performed research; H.K. contributed unpublished reagents/analytic tools; L.J.H., C.P., S.U., and D.S. analyzed data; L.J.H., C.P., and C.J.W. wrote the paper.

  2. ↵* L.J.H. and C.P. contributed equally to this work.

  • C. J. Wierenga’s present address: Faculty of Science and Donders Institute, Radboud University, Nijmegen, The Netherlands.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by the ZonMW TOP Grant 9126021, the Foundation for Fundamental Research on Matter (FOM) Research Program Grant 16NEPH05, and the Dutch Research Council (NWO) Open Competition Program Grant OCENW.KLEIN.150.

Funding

  • ZonMw (Netherlands Organisation for Health Research and Development)

    TOP grant 9126021
  • Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO)

    16NEPH05; OCENW.KLEIN.150

Other Version

  • You are viewing the most recent version of this article.
  • previous version (December 07, 2022).

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eneuro: 9 (6)
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November/December 2022
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Using SuperClomeleon to Measure Changes in Intracellular Chloride during Development and after Early Life Stress
Lotte J. Herstel, Carlijn Peerboom, Sten Uijtewaal, Dunya Selemangel, Henk Karst, Corette J. Wierenga
eNeuro 7 December 2022, 9 (6) ENEURO.0416-22.2022; DOI: 10.1523/ENEURO.0416-22.2022

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Using SuperClomeleon to Measure Changes in Intracellular Chloride during Development and after Early Life Stress
Lotte J. Herstel, Carlijn Peerboom, Sten Uijtewaal, Dunya Selemangel, Henk Karst, Corette J. Wierenga
eNeuro 7 December 2022, 9 (6) ENEURO.0416-22.2022; DOI: 10.1523/ENEURO.0416-22.2022
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Keywords

  • chloride
  • FRET sensor
  • GABA shift
  • two-photon microscopy

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