Figure 4. Prenatal THC induces significant alterations in molecular biomarkers in the NAc. Blots are cropped to fit both protein of interest and α-Tubulin for A, C, D (vGlut1 only), and E. All representative Western blottings exhibit alternating VEH, THC. A. Representative Western blotting of dopamine D2R. Densitometry analysis revealed that, at postnatal day (PD) 21 and PD120, only the male PCE rats (n = 8/treatment) have significant decreases in D2R expression relative VEH controls, while female progeny (n = 8/treatment) exhibited no difference. B, Representative Western blotting of GAD67 at PD120. The male PCE progeny at PD120 exhibits a significant decrease, while the female progeny exhibits a significant increase in GAD67 expression relative to vehicle controls. C, Representative Western blotting of NMDAR2B. At PD21, male progeny exhibits a significant increase, but at PD120, only the female PCE progeny exhibits significant increases in NMDAR2B expression. D, Representative Western blottings of vGlut1 and vGlut2 at PD120. The male and female PCE progeny exhibits no significant deficits in vGlut1, but both exhibit significant increases in vGlut2. E, Representative Western blotting of PPARα at PD120. The female PCE progeny exhibits a significant increase in PPARα expression, while the males exhibit no difference in expression. F, Representative Western blotting of PPAR ɣ1 and ɣ2 at PD120. The female PCE progeny exhibits a significant decrease in PPAR ɣ1 and ɣ2 expression, while the males exhibit no difference in expression. Comparisons were made with two-way ANOVA followed by Tukey’s HSD post hoc test, ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05. Figure Contributions: Mohammed H. Sarikahya, Karen KW Wong, Mar Rodríguez-Ruiz, Tsun Hay Jason Ng, Dana Gummerson, and Emma Proud performed the experiments. Mohammed H. Sarikahya and Karen KW Wong analyzed the experiments.