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Research ArticleResearch Article: Methods/New Tools, Novel Tools and Methods

Measuring Nonapoptotic Caspase Activity with a Transgenic Reporter in Mice

P. J. Nicholls, Thomas F. Pack, Nikhil M. Urs, Sunil Kumar, Yang Zhou, Gabriel Ichim, Joshua D. Ginzel, Gabor Turu, Evan Calabrese, Wendy L. Roberts, Ping Fan, Valeriy G. Ostapchenko, Monica S. Guzman Lenis, Flavio Beraldo, Jiri Hatina, Vania F. Prado, Marco A. M. Prado, Ivan Spasojevic, Joshua C. Snyder, Kafui Dzirasa, G. Allan Johnson and Marc G. Caron
eNeuro 8 September 2022, 9 (5) ENEURO.0147-21.2022; https://doi.org/10.1523/ENEURO.0147-21.2022
P. J. Nicholls
1Department of Psychiatry and Behavioral Sciences, Duke University Medical Center, Durham, NC 27710
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Thomas F. Pack
2Department of Cell Biology, Duke University Medical Center, Durham, NC 27710
3Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710
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Nikhil M. Urs
2Department of Cell Biology, Duke University Medical Center, Durham, NC 27710
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Sunil Kumar
1Department of Psychiatry and Behavioral Sciences, Duke University Medical Center, Durham, NC 27710
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Yang Zhou
2Department of Cell Biology, Duke University Medical Center, Durham, NC 27710
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Gabriel Ichim
10Center for Research on Cancer in Lyon, University of Lyon, Lyon 69008, France
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Joshua D. Ginzel
2Department of Cell Biology, Duke University Medical Center, Durham, NC 27710
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Gabor Turu
2Department of Cell Biology, Duke University Medical Center, Durham, NC 27710
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Evan Calabrese
4Department of Biomedical Engineering, Duke University Medical Center, Durham, NC 27710
5Center for In Vivo Microscopy, Department of Radiology, Duke University Medical Center, Durham, NC 27710
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Wendy L. Roberts
2Department of Cell Biology, Duke University Medical Center, Durham, NC 27710
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Ping Fan
6Department of Medicine, Duke University Medical Center, Durham, NC 27710
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Valeriy G. Ostapchenko
11Robarts Research Institute, University of Western Ontario, London, Ontario N6A 3K7, Canada
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Monica S. Guzman Lenis
11Robarts Research Institute, University of Western Ontario, London, Ontario N6A 3K7, Canada
12Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 3K7, Canada
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Flavio Beraldo
11Robarts Research Institute, University of Western Ontario, London, Ontario N6A 3K7, Canada
13Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario N6A 3K7, Canada
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Jiri Hatina
14Institute of Biology, Charles University, Plzen CZ-32300, Czech Republic
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Vania F. Prado
11Robarts Research Institute, University of Western Ontario, London, Ontario N6A 3K7, Canada
12Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 3K7, Canada
13Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario N6A 3K7, Canada
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Marco A. M. Prado
11Robarts Research Institute, University of Western Ontario, London, Ontario N6A 3K7, Canada
12Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 3K7, Canada
13Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario N6A 3K7, Canada
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Ivan Spasojevic
6Department of Medicine, Duke University Medical Center, Durham, NC 27710
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Joshua C. Snyder
2Department of Cell Biology, Duke University Medical Center, Durham, NC 27710
9Department of Surgery, Duke University Medical Center, Durham, NC 27710
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Kafui Dzirasa
1Department of Psychiatry and Behavioral Sciences, Duke University Medical Center, Durham, NC 27710
4Department of Biomedical Engineering, Duke University Medical Center, Durham, NC 27710
7Department of Neurobiology, Duke University Medical Center, Durham, NC 27710
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G. Allan Johnson
4Department of Biomedical Engineering, Duke University Medical Center, Durham, NC 27710
5Center for In Vivo Microscopy, Department of Radiology, Duke University Medical Center, Durham, NC 27710
8Department of Physics, Duke University, Durham, NC 27710
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Marc G. Caron
2Department of Cell Biology, Duke University Medical Center, Durham, NC 27710
6Department of Medicine, Duke University Medical Center, Durham, NC 27710
7Department of Neurobiology, Duke University Medical Center, Durham, NC 27710
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Measuring Nonapoptotic Caspase Activity with a Transgenic Reporter in Mice
P. J. Nicholls, Thomas F. Pack, Nikhil M. Urs, Sunil Kumar, Yang Zhou, Gabriel Ichim, Joshua D. Ginzel, Gabor Turu, Evan Calabrese, Wendy L. Roberts, Ping Fan, Valeriy G. Ostapchenko, Monica S. Guzman Lenis, Flavio Beraldo, Jiri Hatina, Vania F. Prado, Marco A. M. Prado, Ivan Spasojevic, Joshua C. Snyder, Kafui Dzirasa, G. Allan Johnson, Marc G. Caron
eNeuro 8 September 2022, 9 (5) ENEURO.0147-21.2022; DOI: 10.1523/ENEURO.0147-21.2022

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Measuring Nonapoptotic Caspase Activity with a Transgenic Reporter in Mice
P. J. Nicholls, Thomas F. Pack, Nikhil M. Urs, Sunil Kumar, Yang Zhou, Gabriel Ichim, Joshua D. Ginzel, Gabor Turu, Evan Calabrese, Wendy L. Roberts, Ping Fan, Valeriy G. Ostapchenko, Monica S. Guzman Lenis, Flavio Beraldo, Jiri Hatina, Vania F. Prado, Marco A. M. Prado, Ivan Spasojevic, Joshua C. Snyder, Kafui Dzirasa, G. Allan Johnson, Marc G. Caron
eNeuro 8 September 2022, 9 (5) ENEURO.0147-21.2022; DOI: 10.1523/ENEURO.0147-21.2022
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Keywords

  • caspases
  • in vivo reporter
  • nonapoptotic
  • mapping
  • amygdala
  • stress
  • sex differences

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