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Research ArticleResearch Article: Negative Results, Disorders of the Nervous System

Investigation of MicroRNA-134 as a Target against Seizures and SUDEP in a Mouse Model of Dravet Syndrome

Rogério R. Gerbatin, Joana Augusto, Gareth Morris, Aoife Campbell, Jesper Worm, Elena Langa, Cristina R. Reschke and David C. Henshall
eNeuro 9 September 2022, 9 (5) ENEURO.0112-22.2022; DOI: https://doi.org/10.1523/ENEURO.0112-22.2022
Rogério R. Gerbatin
1Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland
2FutureNeuro SFI Research Centre, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland
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Joana Augusto
1Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland
4Department of Physiology, Faculty of Medicine, Trinity College Dublin, Dublin, D02 PN40, Ireland
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Gareth Morris
1Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland
2FutureNeuro SFI Research Centre, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland
5Department of Neuroscience, Physiology and Pharmacology, University College London, London, WC1E 6BT, United Kingdom
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Aoife Campbell
1Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland
2FutureNeuro SFI Research Centre, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland
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Jesper Worm
6Roche Innovation Center Copenhagen, Copenhagen, CH-4070, Denmark
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Elena Langa
1Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland
2FutureNeuro SFI Research Centre, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland
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Cristina R. Reschke
1Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland
2FutureNeuro SFI Research Centre, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland
3School of Pharmacy and Biomedical Sciences, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland
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David C. Henshall
1Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland
2FutureNeuro SFI Research Centre, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0112-22.2022
PubMed 
36240080
Published By 
Society for Neuroscience
History 
  • Received March 16, 2022
  • Revision received July 31, 2022
  • Accepted August 10, 2022
  • Published online September 9, 2022.
Copyright & Usage 
Copyright © 2022 Gerbatin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Rogério R. Gerbatin1,2,
  2. Joana Augusto1,4,
  3. Gareth Morris1,2,5,
  4. Aoife Campbell1,2,
  5. Jesper Worm6,
  6. Elena Langa1,2,
  7. Cristina R. Reschke1,2,3,* and
  8. David C. Henshall1,2,*
  1. 1Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland
  2. 2FutureNeuro SFI Research Centre, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland
  3. 3School of Pharmacy and Biomedical Sciences, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland
  4. 4Department of Physiology, Faculty of Medicine, Trinity College Dublin, Dublin, D02 PN40, Ireland
  5. 5Department of Neuroscience, Physiology and Pharmacology, University College London, London, WC1E 6BT, United Kingdom
  6. 6Roche Innovation Center Copenhagen, Copenhagen, CH-4070, Denmark
  1. Correspondence should be addressed to David C. Henshall at dhenshall{at}rcsi.ie.
View Full Text

Author contributions

  1. Author contributions: D.C.H., R.R.G., J.W., and C.R.R. designed research; D.C.H. contributed unpublished reagents/analytic tools; R.R.G., J.A., G.M., A.C., E.L., and C.R.R. performed research; R.G. analyzed data; R.R.G. wrote the paper.

  2. ↵* C.R.R. and D.C.H. contributed equally to this work.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by the Science Foundation Ireland (SFI) Grant 16/RC/3948, the European Regional Development Fund, FutureNeuro industry partners, the Charitable Infirmary Charitable Trust Grant 108, the Marie Skłodowska-Curie Actions Grant H2020-MSCA-IF-2018 840262 (to G.M.), the Emerging Leader Fellowship Award F2102 Morris, and F. Hoffman-La Roche Ltd. C.R.R. was supported by CURE epilepsy.

Funding

  • Science Fundation Ireland (SFI)

    16/RC/3948
  • Charitable Infirmary Charitable Trust

    Grant 108
  • Marie Skłodowska-Curie Actions

    H2020-MSCA-IF-2018 840262
  • Emerging Leader Fellowship Award

    F2102 Morris

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  • previous version (September 09, 2022).

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Investigation of MicroRNA-134 as a Target against Seizures and SUDEP in a Mouse Model of Dravet Syndrome
Rogério R. Gerbatin, Joana Augusto, Gareth Morris, Aoife Campbell, Jesper Worm, Elena Langa, Cristina R. Reschke, David C. Henshall
eNeuro 9 September 2022, 9 (5) ENEURO.0112-22.2022; DOI: 10.1523/ENEURO.0112-22.2022

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Investigation of MicroRNA-134 as a Target against Seizures and SUDEP in a Mouse Model of Dravet Syndrome
Rogério R. Gerbatin, Joana Augusto, Gareth Morris, Aoife Campbell, Jesper Worm, Elena Langa, Cristina R. Reschke, David C. Henshall
eNeuro 9 September 2022, 9 (5) ENEURO.0112-22.2022; DOI: 10.1523/ENEURO.0112-22.2022
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Keywords

  • Dravet syndrome
  • miR-134
  • Oligonucleotides
  • seizure
  • SUDEP

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