Extended Data Figure 1-2
Pluripotency characterization of XDP female carrier-derived iPSCs. a, Bulk RNA-sequencing analysis revealed that all XDP female carrier-derived iPSC clones expressed multiple markers associated with pluripotency, such as SOX2, POU5F1, and NANOG; they did not express genes associated with three germ layers: EOMES (mesoderm), GATA4 (endoderm), and PAX6 (neuroectoderm). b, Expression of the pluripotency markers NANOG (green) and TRA-1-60 (red) was detected by immunofluorescence staining in all eight XDP carrier iPSCs. Hoechst (blue) was used to visualize nuclei. Merged images depict overlays of immunoreactivity for each target, together with the nuclear counterstain. Scale bar: 20 μm. c, Comparative expression of primed (DUSP6, DNMT3A, SOX11) and naive (DPPA5, KLF17, DNMT3L) markers in all eight XDP female carrier-derived iPSCs. d, Representative images from isogenic XDP female carrier iPSC lines showing localization of RNA scope-specific probes for DUSP6 (green) transcripts. DAPI was used to stain nuclei. Scale bar: 20 μm. Figure contribution: Bareera Qamar performed the fluorescence immunostaining on iPSCs. Laura D’Ignazio performed the RNA scope assay. Kynon J. M. Benjamin performed the RNA-seq data analysis. Download Figure 1-2, TIF file.