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Research ArticleResearch Article: Methods/New Tools, Novel Tools and Methods

Duplex Labeling and Manipulation of Neuronal Proteins Using Sequential CRISPR/Cas9 Gene Editing

Wouter J. Droogers, Jelmer Willems, Harold D. MacGillavry and Arthur P. H. de Jong
eNeuro 18 July 2022, 9 (4) ENEURO.0056-22.2022; https://doi.org/10.1523/ENEURO.0056-22.2022
Wouter J. Droogers
Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, 3584 CH, Utrecht, The Netherlands
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Jelmer Willems
Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, 3584 CH, Utrecht, The Netherlands
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Harold D. MacGillavry
Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, 3584 CH, Utrecht, The Netherlands
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Arthur P. H. de Jong
Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, 3584 CH, Utrecht, The Netherlands
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0056-22.2022
PubMed 
35851300
Published By 
Society for Neuroscience
History 
  • Received February 6, 2022
  • Revision received April 20, 2022
  • Accepted April 28, 2022
  • Published online July 18, 2022.
Copyright & Usage 
Copyright © 2022 Droogers et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Wouter J. Droogers,
  2. Jelmer Willems,
  3. Harold D. MacGillavry* and
  4. Arthur P. H. de Jong*
  1. Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, 3584 CH, Utrecht, The Netherlands
  1. Correspondence should be addressed to Harold D. MacGillavry at h.d.macgillavry{at}uu.nl or Arthur P. H. de Jong at a.p.h.dejong{at}uu.nl.
  • ↵* H.D.M. and A.P.H.d.J. contributed equally to this work.

View Full Text

Author contributions

  1. Author contributions: H.D.M. and A.P.H.d.J. designed research; W.J.D., J.W., and A.P.H.d.J. performed research; W.J.D., J.W., and A.P.H.d.J. contributed unpublished reagents/analytic tools; W.J.D., J.W., and A.P.H.d.J. analyzed data; A.P.H.d.J. wrote the paper.

  2. A.P.H. de Jong’s present address: Scenic Biotech, 1098 XG, Amsterdam, The Netherlands.

  • ↵* H.D.M. and A.P.H.d.J. contributed equally to this work.

View Abstract

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by the European Research Council Grant ERC-StG 716011 (to H.D.M.), the Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO) Grant OCENW.KLEIN.163 (to H.D.M.), and a NARSAD Young Investigator Grant from the Brain & Behavior Research Foundation (Grant 29452; to A.P.H.d.J.).

Funding

  • EC | European Research Council (ERC)

    ERC-StG 716011
  • Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO)

    OCENW.KLEIN.163
  • Brain and Behavior Research Foundation (BBRF)

    29452

Other Version

  • You are viewing the most recent version of this article.
  • previous version (July 18, 2022).

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Jul 2022482283224
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Total 20251941954446
Jan 20261320126
Feb 20262229526
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Apr 202686733
Total 202660787147
Total125493152371
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eneuro: 9 (4)
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Duplex Labeling and Manipulation of Neuronal Proteins Using Sequential CRISPR/Cas9 Gene Editing
Wouter J. Droogers, Jelmer Willems, Harold D. MacGillavry, Arthur P. H. de Jong
eNeuro 18 July 2022, 9 (4) ENEURO.0056-22.2022; DOI: 10.1523/ENEURO.0056-22.2022

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Duplex Labeling and Manipulation of Neuronal Proteins Using Sequential CRISPR/Cas9 Gene Editing
Wouter J. Droogers, Jelmer Willems, Harold D. MacGillavry, Arthur P. H. de Jong
eNeuro 18 July 2022, 9 (4) ENEURO.0056-22.2022; DOI: 10.1523/ENEURO.0056-22.2022
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Keywords

  • CRISPR/Cas9
  • fluorescence microscopy
  • knock-in
  • multiplex genome editing
  • neurons

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