Extended Data Figure 5-2
Effect of α-synF on the mobility of Magic Red-labeled lysosomes and their transport in mouse cortical neurons at DIC21, after 24-h exposure to α-synF at concentration of 0.2 μm, compared to nothing added control. A, Number of lysosomes detected per FoV of 40 × 80 μm size during 2 min of observation. B, Fraction of lysosomes having a directed motion. C, Trajectory length. D, Examples of lysosome trajectories. Scale bar: 10 μm. E–H, Comparison of four transport parameters: curvilinear velocity (E), run length (F), pausing time (G), and pausing frequency (H). I, Diameter of Magic Red-labeled lysosomes. General remarks: the number inside the bar represents the total number of FoVs (A, B) and trajectories (E–I). Inset, Box-plots representations of the same datasets (C–I). Download Figure 5-2, TIF file.